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Cholesterol biosynthesis. Metabolism of 14(alpha)-hydroxymethly-5-(alpha)-cholest-7-en-3(beta)-ol
[3a- H]-14a-Hydroxymethyl-5a-choiest-7-en-3B-ol, prepared by chemical synthesis, was incubated with rat liver homogenate preparations (1, x g supernatant fraction) . Efficient conversion to cholesterol was observed. Column ...
Modification of lactose repressor protein with 2-chloromercuri-4-nitrophenol
The reaction of the lactose repressor protein of Escherichia coli with 2-chloromercuri-4-nitrophenol (MNP) has been investigated. MNP reacts specifically with thiol groups in proteins. When a thiol reacts with a ...
Phosphorylated derivatives of dihydrostreptomycin produced by Streptomyces glebosus
The filamentous soil "bacterium, Streptomyces hygrosooplcus forma glebosus ATCC 1467 ( S. glebosus ), synthesizes the antibiotic bluensomycln, a monoguanidinated analogue of dihydrostreptomycin (DSM) Cell-free extracts of’ ...
Inhibition of rat liver pyruvate carboxylase by inert chromium-nucleotide coordination complexes
Pyruvate carboxylase, Pyruvate:CO2 Ligase (ADP); EC 22.214.171.124, free of contaminating enzymatic activity and suitable for kinetic studies, was purified from rat liver. Protomers of the enzyme, rather than tetramers, were ...