Now showing items 1-6 of 6
Experimental evolution of TetX2: Correlating changes in fitness to their structural and functional origins
The study of protein evolution and adaptation resides at the junction between the disciplines of biological chemistry and evolutionary biology. We chose the B. thetaiotaomicron tetracycline resistant enzyme TetX2, as our ...
The BIophysical Basis for Adaptation: Predicting Evolutionary Outcomes from Physicochemical Properties
Experimental evolution can be used in conjunction with biophysical characterization of enzymes to determine the link between cellular fitness and physicochemical properties of enzymes. Sequencing of ancestral and evolved ...
Evolutionary fates within a microbial population highlight an essential role for protein folding during natural selection
The fitness function developed in this thesis directly links the physicochemical properties of an enzyme to evolutionary fates in a quantitative and predictive manner through a comparative study of empirical and simulated ...
A Novel Phosphodiesterase of the GdpP Family Modulates Cyclic di-AMP Levels in Response to Cell Membrane Stress in Daptomycin-Resistant Enterococci
Antimicrobial resistance is a serious and growing threat to public health. The Centers for Disease Control estimates that at least 2 million people contract serious infections each year resulting in at least 23,000 deaths ...
Evolutionary Dynamics Leading to Tigecycline Resistance in Enterococcus faecalis
The rising frequency of multidrug resistant pathogens poses an increasing threat to public health. There is a dire need for novel approaches to combat these deadly pathogens and maintain the efficacy of currently available ...
Cellular fitness as a proxy to determine the physicochemical parameters of an antibiotic efflux pump
Determining the quantitative link between protein function and cellular fitness can be challenging as even the modestly sized genome of Escherichia coli is comprised of thousands of genes. Using an appropriate survey of ...