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Enhancing Stability and Expression of Recombinant Human Hemoglobin in E. Coli: Progress in the Development of a Recombinant HBOC Source
The commercial feasibility of recombinant human Hb (rHb) as an O2 delivery pharmaceutical is limited by the production yield of holoprotein in E. coli. Currently the production ofrHb is not cost effective for use as a ...
AprA: An autocrine, secreted factor that represses cell proliferation in Dictyostelium discoideum
Dictyostelium cells secrete factors during growth and starvation. AprA (autocrine proliferation repressor), a 60 kDa protein secreted as part of a 150 kDa protein complex, functions as a cell proliferation repressor. In ...
Monitoring iron-sulfur cluster content of human glutaredoxin 2 using fluorescence proteins
Current methods for directly determining whether an iron-sulfur cluster has been synthesized on a protein in vivo typically require that the protein of interest is purified prior to analysis for bound metallocluster. To ...
Molecular genetic analyses of plant peroxisome functions
Arabidopsis peroxisomes carry out diverse functions, including fatty acid β-oxidation and conversion of indole-3-butyric acid (IBA) to indole-3-acetic acid (IAA). Peroxins are proteins necessary for peroxisome biogenesis; ...