Now showing items 1-10 of 304
Ligand and genetic studies of the sulfate-binding protein and characterization of the calcium-binding site in the galactose-binding protein
The effect of pH on the binding affinities of the conjugate bases of four different tetrahedral oxyacids to the periplasmic sulfate-binding protein from Salmonella typhimurium has been determined. In light of the highly ...
Hypertonic shock and the cell cycle: Identification of a stress-induced G2 delay in Saccharomyces cerevisiae
Exposure of S. cerevisiae cells to an increase in external osmolarity induces a temporary growth arrest. Recovery from this stress is mediated by the accumulation of intracellular glycerol and the transcription of several ...
Genes expressed during oogenesis in Calliphora erythrocephala and Drosophila melanogaster
We were interested in identifying genes in the dipteran flies Calliphora erythrocephala and Drosophila melanogaster with a role in oogenesis and early embryogenesis. A biochemical screen was used to complement the extensive ...
A simple and effective model to study insulin resistance in obesity and diabetes and the processes and mechanisms of in vitro aging using lymphocyte culture
Cell culture provides a simple and effective system to study metabolic and cellular processes required for cellular growth in vitro. In this study, we demonstrate that lymphocyte culture can be used as a model system to ...
Analysis of HeLa cell premessenger RNA splicing complexes containing the snRNP U1 by native gel electrophoresis
The typical eukaryotic RNA polymerase II primary transcript is divided into regions that encode information expressed at the protein level (exons) and those which do not (introns). The latter must be removed from the ...
Studies on Escherichia coli tryptophan repressor-operator interactions
Trp repressor regulates the expression of TrpEDCBA, aroH, and TrpR operons. Recently, X-ray crystallographic analysis of a Trp repressor-operator complex has suggested a stoichiometry of one repressor dimer to one operator ...
The vascular system of the rabbit ovary and its relationship to ovulation
Abstract Not Available.