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Nanoshells in vivo imaging using two-photon excitation microscopy
This thesis describes the development and optical characterization of near infrared (NIR) gold nanoshells for the use as luminescent contrast agents for applications in small animal blood vessel imaging. Two types of gold-silica nanoshells excitable by NIR lasers are investigated: Type 1 nanoshells can be excited with a sub-mum NW laser, whereas Type ...
Development of image mapping spectrometer (IMS) for hyperspectral fluorescence microscopy
An image mapping spectrometer (IMS) for microscopy applications is presented. Its principle is based on the redirecting of image zones by specially organized thin mirrors within a custom fabricated component termed an image mapper. The demonstrated prototype can simultaneously acquire a 140nm spectral range within its 2D field of view from a single ...