The elucidation of the molecular mechanisms of platelet adhesion and aggregation in response to naturally occurring subendothelial surfaces under flow conditions is important medically in atherosclerosis and mural thrombosis. Type VI collagen is a subendothelial constituent that binds vWF and platelets. The interaction of platelets with type VI ...

Part of the definition given to the new emerging science discipline of tissue engineering is the understanding of the structure-function relationships at the cellular level. In this context it is important for vascular tissue engineering to understand the mechanisms involved in the vascular cell responses to their mechanically active environment. ...

This study aims to determine the effect of shear stress on membrane potassium (K$\sp+$) permeability in vascular endothelial cells. Cultured monolayers of calf pulmonary artery endothelial cells, preloaded with $\rm \sp{86}Rb\sp+$, were subjected to different levels of fluid shear stress in the range 1-10 dynes/cm$\sp2$, in a parallel-plate geometry ...

Epi-fluorescence video microscopy and digital image processing were used to evaluate the effects of three novel anti-platelet agents namely GT-12/I.2HBr (GT-12/I), A4.2HBr and IC, a series of compounds containing the $-$3-carboxylic acid piperidine moiety as their functional group, on platelet aggregation. GT-12/I was evaluated at a concentration of ...

Endothelial cell biology has become a major area of research during the last decade. An increasing body of evidence suggests that the local mechanical forces arising from blood flow play a key role in the normal physiology and pathobiology of the endothelium. In this study, monolayers of human endothelial cells were subjected to hydrodynamically-induced ...

Neutrophil adhesion to endothelium is prerequisite for extravasation and localization at inflammatory sites. In the vasculature this process occurs under conditions of hydrodynamic shear requiring that bonds formed between neutrophils and endothelium withstand forces imposed by flowing blood. In this work we investigated interactions of neutrophils ...

Sickle erythrocytes have previously been demonstrated to be abnormally adhesive to human endothelial cells in both static incubation assays and under venous flow conditions. The extent to which human fibronectin and human von Willebrand factor (vWF) can promote sickle erythrocyte adhesion to human endothelial cells was studied under venous flow ...

The effect of flow on the adhesion of neutrophils (PMNL) to vascular endothelium was investigated using a parallel plate flow chamber. Human umbilical vein endothelial cells (HUVEC) were cultured on a glass slide which was fitted onto the flow cell. Formyl-methionyl-leucyl-phenyl-alanine (FMLP), a chemotactic tripeptide, interleukin-1 (IL1), ...

Two constitutive equations for viscoelastic fluids are examined in linearized hydrodynamic stability analysis for the effects of viscous dissipation, in plane Couette flow heated from below. All physical properties are assumed constant except the density in the body-force term of the momentum equation. Critical and marginal Rayleigh numbers are ...

Epi-fluorescent video microscopy was used to evaluate the effect of ethanol and two nipecotoylpiperazine based antiplatelet agents (BPAT-117 and BPAT-143) on platelet mural thrombus formation. In addition, a series of novel radio frequency glow discharge (RFGD) modified surfaces with varying nitrogen content were evaluated for blood biocompatibility. Whole ...