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Experimental evolution of TetX2: Correlating changes in fitness to their structural and functional origins
The study of protein evolution and adaptation resides at the junction between the disciplines of biological chemistry and evolutionary biology. We chose the B. thetaiotaomicron tetracycline resistant enzyme TetX2, as our ...
The BIophysical Basis for Adaptation: Predicting Evolutionary Outcomes from Physicochemical Properties
Experimental evolution can be used in conjunction with biophysical characterization of enzymes to determine the link between cellular fitness and physicochemical properties of enzymes. Sequencing of ancestral and evolved ...
Evolutionary fates within a microbial population highlight an essential role for protein folding during natural selection
The fitness function developed in this thesis directly links the physicochemical properties of an enzyme to evolutionary fates in a quantitative and predictive manner through a comparative study of empirical and simulated ...