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dc.contributor.authorChen, Jixin
Poddar, Nitesh K.
Tauzin, Lawrence J.
Cooper, David
Kolomeisky, Anatoly B.
Landes, Christy F.
dc.date.accessioned 2015-10-29T18:23:24Z
dc.date.available 2015-10-29T18:23:24Z
dc.date.issued 2014
dc.identifier.citation Chen, Jixin, Poddar, Nitesh K., Tauzin, Lawrence J., et al.. "Single-Molecule FRET Studies of HIV TAR–DNA Hairpin Unfolding Dynamics." The Journal of Physical Chemistry B, 118, no. 42 (2014) American Chemical Society: 12130-12139. http://dx.doi.org/10.1021/jp507067p.
dc.identifier.urihttps://hdl.handle.net/1911/81984
dc.description.abstract We directly measure the dynamics of the HIV trans-activation response (TAR)-DNA hairpin with multiple loops using single-molecule Förster resonance energy transfer (smFRET) methods. Multiple FRET states are identified that correspond to intermediate melting states of the hairpin. The stability of each intermediate state is calculated from the smFRET data. The results indicate that hairpin unfolding obeys a "fraying and peeling" mechanism, and evidence for the collapse of the ends of the hairpin during folding is observed. These results suggest a possible biological function for hairpin loops serving as additional fraying centers to increase unfolding rates in otherwise stable systems. The experimental and analytical approaches developed in this article provide useful tools for studying the mechanism of multistate DNA hairpin dynamics and of other general systems with multiple parallel pathways of chemical reactions.
dc.language.iso eng
dc.publisher American Chemical Society
dc.rights This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
dc.rights.urihttp://pubs.acs.org/page/policy/authorchoice_termsofuse.html
dc.title Single-Molecule FRET Studies of HIV TAR–DNA Hairpin Unfolding Dynamics
dc.type Journal article
dc.contributor.funder Welch Foundation
dc.contributor.funder National Science Foundation
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle The Journal of Physical Chemistry B
dc.citation.volumeNumber 118
dc.citation.issueNumber 42
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1021/jp507067p
dc.identifier.pmcid PMC4207534
dc.identifier.pmid 25254491
dc.identifier.grantID C-1787 (Welch Foundation)
dc.identifier.grantID CBET-1133965 (National Science Foundation)
dc.identifier.grantID CHE-1151647 (National Science Foundation)
dc.identifier.grantID GM94246-01A1 (National Institutes of Health)
dc.type.publication publisher version
dc.citation.firstpage 12130
dc.citation.lastpage 12139


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