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    Single-Molecule FRET Studies of HIV TAR–DNA Hairpin Unfolding Dynamics

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    Author
    Chen, Jixin; Poddar, Nitesh K.; Tauzin, Lawrence J.; Cooper, David; Kolomeisky, Anatoly B.; More... Landes, Christy F. Less...
    Date
    2014
    Abstract
    We directly measure the dynamics of the HIV trans-activation response (TAR)-DNA hairpin with multiple loops using single-molecule Förster resonance energy transfer (smFRET) methods. Multiple FRET states are identified that correspond to intermediate melting states of the hairpin. The stability of each intermediate state is calculated from the smFRET data. The results indicate that hairpin unfolding obeys a "fraying and peeling" mechanism, and evidence for the collapse of the ends of the hairpin during folding is observed. These results suggest a possible biological function for hairpin loops serving as additional fraying centers to increase unfolding rates in otherwise stable systems. The experimental and analytical approaches developed in this article provide useful tools for studying the mechanism of multistate DNA hairpin dynamics and of other general systems with multiple parallel pathways of chemical reactions.
    Citation
    Chen, Jixin, Poddar, Nitesh K., Tauzin, Lawrence J., et al.. "Single-Molecule FRET Studies of HIV TAR–DNA Hairpin Unfolding Dynamics." The Journal of Physical Chemistry B, 118, no. 42 (2014) American Chemical Society: 12130-12139. http://dx.doi.org/10.1021/jp507067p.
    Published Version
    http://dx.doi.org/10.1021/jp507067p
    Type
    Journal article
    Publisher
    American Chemical Society
    Citable link to this page
    https://hdl.handle.net/1911/81984
    Rights
    This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
    Link to License
    http://pubs.acs.org/page/policy/authorchoice_termsofuse.html
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    • Chemistry Publications [636]
    • ECE Publications [1443]
    • Faculty Publications [4990]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map