Reduced phosphotransferase system activity in bacteria
AuthorSan, Ka-Yiu; Bennett, George N.; Causey, Thomas Bryce; Wong, Matthew Sing; Wu, Steven
A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI bacteria produce large quantities of recombinant protein without producing significant amounts of acetate. The bacteria grow well on standard LB broth without additional supplementation.
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- Rice Patents