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dc.creatorBennett, George N.
Harrison, Mary Lou
dc.date.accessioned 2015-05-04T19:05:51Z
dc.date.available 2015-05-04T19:05:51Z
dc.date.issued 2009-12-08
dc.identifier.urihttp://hdl.handle.net/1911/80002
dc.description.abstract A process for assembling a series of DNA fragments generated by PCR into an ordered circular arrangement for replication and genetic work in cells. The PCR fragments are made with a modified nucleotide in the primers that can be removed with a DNA excision repair enzyme to generate a 3′ overhang. The 3′ overhangs are designed to allow directional annealing and thus sequential PCR fragments can be assembled by annealing the overhangs and subsequent ligation. Sequential addition of PCR fragments is facilitated by growing the chain on a solid support, and the assembled chain can be removed with a site specific recombinase if the first and last primers contain the recombinase site. The circularized assembled fragment can be directly used for cell transformation if the appropriate sequences are included, such as an origin of replication and a selectable marker.
dc.format.extent 13 pp
dc.language.iso eng
dc.title Method for assembling PCR fragments of DNA
dc.type Utility patent
dc.digitization.specificationsThis patent information was downloaded from the US Patent and Trademark website (http://www.uspto.gov/) as image-PDFs. The PDFs were OCRed for access purposes.
dc.contributor.publisher United States Patent and Trademark Office
dc.type.genre patents
dc.type.dcmi Text
dc.date.filed 2003-10-31
dc.identifier.patentID US7629120B2
dc.contributor.assignee Rice University
dc.identifier.citation Bennett, George N. and Harrison, Mary Lou, "Method for assembling PCR fragments of DNA." Patent US7629120B2. issued 2009-12-08. Retrieved from http://hdl.handle.net/1911/80002.


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