Now showing items 1-19 of 19

    • Aerobic succinate production in bacteria 

      San, Ka-Yiu; Bennett, George N.; Lin, Henry (2007-07-17)
      Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
    • Aerobic succinate production in bacteria 

      San, Ka-Yiu; Bennett, George N.; Lin, Henry (2007-08-28)
      Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
    • Aerobic succinate production in bacteria 

      San, Ka-Yiu; Bennett, George N.; Lin, Henry (2011-05-03)
      Methods of increasing yields of succinate using aerobic culture methods and a multi-mutant E. coli strain are provided. Also provided is a mutant strain of E. coli that produces high amounts of succinic acid.
    • Engineered bacteria produce succinate from sucrose 

      San, Ka-Yiu; Bennett, George N.; Wang, Jian (2015-02-24)
      Bacteria optimized to produce succinate and other feedstocks by growing on low cost carbon sources, such as sucrose.
    • High molar succinate yield bacteria by increasing the intracellular NADH availability 

      San, Ka-Yiu; Bennett, George N.; Sánchez, Ailen (2011-04-19)
      The invention relates to increasing the yield of succinate in bacteria by increasing the intracellular availability of cofactors such as NADH.
    • Increased bacterial CoA and acetyl-CoA pools 

      San, Ka-Yiu; Bennett, George N.; Vadali, Ravishankar V. (2011-03-08)
      Methods of increasing the cellular pool of A-CoA and thus driving the metabolic pathways in the direction of A-CoA containing metabolites by overexpressing rate limiting enzymes in A-CoA synthesis. Methods of increasing ...
    • Increasing bacterial succinate productivity 

      San, Ka-Yiu; Bennett, George N.; Balzer, Grant; Zhu, Jiang; Thakker, Chandresh; Sánchez, Ailen (2014-08-05)
      Improved bacteria for making succinate and other 4 carbon dicarboxylates from the Krebs cycle have modifications to reduce acetate, lactate, EtOH and formate, as well as turn on the glyoxylate shunt, produce more NADH and ...
    • Increasing intracellular NADPH availability in E. coli 

      San, Ka-Yiu; Bennett, George N.; Sánchez, Ailen (2008-02-05)
      A method of increasing cellular NADPH levels by expressing one or more genes that encode a enzyme that causes the production of NADPH. The system can be combined with other enzymes that require NADPH, thus improving the ...
    • Increasing NADPH-dependent products 

      San, Ka-Yiu; Bennett, George N.; Lin, Henry; Martinez, Irene; Zhu, Jiang (2014-12-09)
      A method of increasing cellular NADPH levels by expressing one or more genes that encode an enzyme that causes the production of NADPH. The system is combined with other enzymes that require NADPH, thus improving the overall ...
    • Large scale microbial culture method 

      Segueilha, Laurent; San, Ka-Yiu; Bennett, George N.; Martinez, Irene (2013-07-16)
      A new culture method for producing high levels of a metabolite, such as succinic acid uses oxygen rich culture without pH adjustment to increase the biomass, acclimation in under oxygen lean conditions having
    • Mutant E. coli strain with increased succinic acid production 

      San, Ka-Yiu; Bennett, George N.; Sánchez, Ailen (2010-09-07)
      The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.
    • Mutant E. coli strain with increased succinic acid production 

      San, Ka-Yiu; Bennett, George N.; Sánchez, Ailen (2007-05-29)
      The invention relates to a mutant strain of bacteria, which either lacks or contains mutant genes for several key metabolic enzymes, and which produces high amounts of succinic acid under anaerobic conditions.
    • Native NAD-dependent GAPDH replaced with NADP-dependent GAPDH plus NADK 

      San, Ka-Yiu; Bennett, George N.; Wang, Yipeng (2014-04-29)
      This invention is metabolically engineer bacterial strains that provide increased intracellular NADPH availability for the purpose of increasing the yield and productivity of NADPH-dependent compounds. In the invention, ...
    • Natural products from Vinca 

      San, Ka-Yiu; Chung, Ill-Min; Ahmad, Ateeque (2012-06-26)
      New and useful compounds C. roseus cultured hairy roots are provided, by Isolating flavone glycosides together with a pharmaceutically acceptable carrier. This is accomplished by immersing powdered the hairy roots in ...
    • Pantothenate kinase overexpression and pantothenic acid supplementation in actinomycetes 

      Lee, Hei Chan; Sohng, Jae Kyung; San, Ka-Yiu (2012-03-27)
      The invention relates to the synthesis of polyketides in Actinomycetes by overexpression of pantothenate kinase and supplementation with pantothenic acid. This results in increasing in vivo CoA production and thereby drives ...
    • Recycling system for manipulation of intracellular NADH availability 

      San, Ka-Yiu; Berrios-Rivera, Susan J.; Bennett, George N. (2010-05-04)
      The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a ...
    • Recycling system for manipulation of intracellular NADH availability 

      San, Ka-Yiu; Berrios-Rivera, Susan J.; Bennett, George N. (2007-08-14)
      The present invention describes a novel recombinant NADH recycling system that is used as a process for producing reduced compounds. In a specific embodiment, the reduced compounds include ethanol, succinate, lactate, a ...
    • Reduced phosphotransferase system activity in bacteria 

      San, Ka-Yiu; Bennett, George N.; Causey, Thomas Bryce; Wong, Matthew Sing; Wu, Steven (2012-08-07)
      A method of producing biological products using bacteria with an inactivated ptsHI and wild type err and no added glucose transport activity and which consumes nearly all glucose in the media is described. The ΔptsHI ...
    • Simultaneous anaerobic production of isoamyl acetate and succinic acid 

      San, Ka-Yiu; Sánchez, Ailen; Bennett, George N.; Dittrich, Cheryl Renee (2009-08-04)
      In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in ...