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dc.contributor.authorFigueroa, Elizabeth R.
Lin, Adam Y.
Yan, Jiaxi
Luo, Laureen
Foster, Aaron E.
Drezek, Rebekah A.
dc.date.accessioned 2015-03-16T16:56:09Z
dc.date.available 2015-03-16T16:56:09Z
dc.date.issued 2014
dc.identifier.citation Figueroa, Elizabeth R., Lin, Adam Y., Yan, Jiaxi, et al.. "Optimization of PAMAM-gold nanoparticle conjugation for gene therapy." Biomaterials, 35, no. 5 (2014) Elsevier: 1725-1734. http://dx.doi.org/10.1016/j.biomaterials.2013.11.026.
dc.identifier.urihttps://hdl.handle.net/1911/79346
dc.description.abstract The development of efficient and biocompatible non-viral vectors for gene therapy remains a great challenge, and exploiting the properties of both nanoparticle carriers and cationic polymers is an attractive approach. In this work, we have developed gold nanoparticle (AuNP) polyamidoamine (PAMAM) conjugates for use as non-viral transfection agents. AuPAMAM conjugates were prepared by crosslinking PAMAM dendrimers to carboxylic-terminated AuNPs via EDC and sulfo-NHS chemistry. EDC and sulfo-NHS have been utilized widely and in numerous applications such as amino acid coupling; however, their use in the coupling of PAMAM dendrimers to AuNPs presents new challenges to form effective and stable constructs for delivery that have not yet been examined. Enhanced colloidal stability and DNA condensation ability was established by probing two critical synthetic parameters: the reaction rate of the PAMAM crosslinking step, and the amine to carboxyl ratio. Based on this work, increasing the amine to carboxyl ratio during conjugation of PAMAM onto AuNPs yielded the optimal vector with respect to colloidal stability and transfection efficiency inᅠvitro. AuPAMAM conjugates present attractive candidates for non-viral gene delivery due to their commercial availability, ease of fabrication and scale-up, high yield, high transfection efficiency and low cytotoxicity.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Optimization of PAMAM-gold nanoparticle conjugation for gene therapy
dc.type Journal article
dc.contributor.funder Ford Foundation
dc.contributor.funder National Institute of Biomedical Imaging and Bioengineering
dc.contributor.funder Baylor College of Medicine
dc.contributor.funder National Institutes of Health/National Cancer Institute
dc.contributor.funder Welch Foundation
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle Biomaterials
dc.subject.keywordgold
nanoparticle
nanocomposite
gene therapy
dendrimer
DNA
dc.citation.volumeNumber 35
dc.citation.issueNumber 5
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2013.11.026
dc.identifier.pmcid PMC3906732
dc.identifier.pmid 24286816
dc.identifier.grantID pre-doctoral fellowship (Ford Foundation)
dc.identifier.grantID T32EB009379 (National Institute of Biomedical Imaging and Bioengineering)
dc.identifier.grantID Medical Scientist Training Program (Baylor College of Medicine)
dc.identifier.grantID Edward and Josephine Hudson Scholarship (Baylor College of Medicine)
dc.identifier.grantID F30CA165686-01A1 (National Institutes of Health/National Cancer Institute)
dc.identifier.grantID C-1598 (Welch Foundation)
dc.identifier.grantID R01 CA172836 (National Institutes of Health)
dc.type.publication post-print
dc.citation.firstpage 1725
dc.citation.lastpage 1734


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