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dc.contributor.authorTran, Lesa A.
Hernández-Rivera, Mayra
Berlin, Ari N.
Zheng, Yi
Sampaio, Luiz
Bové, Christina
Cabreira-Hansen, Maria da Graça
Willerson, James T.
Perin, Emerson C.
Wilson, Lon J.
dc.date.accessioned 2015-03-16T16:56:09Z
dc.date.available 2015-03-16T16:56:09Z
dc.date.issued 2014
dc.identifier.urihttp://hdl.handle.net/1911/79342
dc.description.abstract In this work, the effectiveness of using Gadonanotubes (GNTs) with an external magnetic field to improve retention of transplanted adult mesenchymal stem cells (MSCs) during cellular cardiomyoplasty was evaluated. As a high-performance T1-weighted magnetic resonance imaging (MRI) cell tracking label, the GNTs are gadolinium-loaded carbon nanotube capsules that render MSCs magnetic when internalized. MSCs were internally labeled with either superparamagnetic GNTs or colloidal diamagnetic lutetium (Lu). In vitro cell rolling assays and ex vivo cardiac perfusion experiments qualitatively demonstrated increased magnetic-assisted retention of GNT-labeled MSCs. Subsequent in vivo epicardial cell injections were performed around a 1.3 T NdFeB ring magnet sutured onto the left ventricle of female juvenile pigs (n = 21). Cell dosage, magnet exposure time, and endpoints were varied to evaluate the safety and efficacy of the proposed therapy. Quantification of retained cells in collected tissues by elemental analysis (Gd or Lu) showed that the external magnet helped retain nearly three times more GNT-labeled MSCs than Lu-labeled cells. The sutured magnet was tolerated for up to 168 h; however, an inflammatory response to the magnet was noted after 48 h. These proof-of-concept studies support the feasibility and value of using GNTs as a magnetic nanoparticle facilitator to improve cell retention during cellular cardiomyoplasty.
dc.language.iso eng
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title The use of gadolinium-carbon nanostructures to magnetically enhance stem cell retention for cellular cardiomyoplasty
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.contributor.funder National Science Foundation
dc.contributor.funder Welch Foundation
dc.citation.journalTitle Biomaterials
dc.contributor.org Smalley Institute for Nanoscale Science and Technology
dc.subject.keywordsingle-walled carbon nanotube
cellular cardiomyoplasty
gadonanotube
mesenchymal stem cell
nanotechnology
dc.citation.volumeNumber 35
dc.citation.issueNumber 2
dc.contributor.publisher Elsevier
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2013.10.013
dc.identifier.pmcid PMC3900574
dc.identifier.pmid 24148239
dc.identifier.grantID 1RC1EB010791-01 (National Institutes of Health)
dc.identifier.grantID DGE-0940902 (National Science Foundation)
dc.identifier.grantID C-0627 (Welch Foundation)
dc.identifier.grantID EEC-0647452 (National Science Foundation)
dc.type.publication post-print
dc.citation.firstpage 720
dc.citation.lastpage 726
dc.identifier.citation Tran, Lesa A., Hernández-Rivera, Mayra, Berlin, Ari N., et al., . "The use of gadolinium-carbon nanostructures to magnetically enhance stem cell retention for cellular cardiomyoplasty." Biomaterials, 35, no. 2 (2014) 720-726. http://dx.doi.org/10.1016/j.biomaterials.2013.10.013.


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