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    Proteomic analyses reveal distinct chromatin‐associated and soluble transcription factor complexes

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    Author
    Li, Xu
    Wang, Wenqi
    Wang, Jiadong
    Malovannaya, Anna
    Xi, Yuanxin
    Li, Wei
    Guerra, Rudy
    Hawke, David H.
    Qin, Jun
    Chen, Junjie
    Date
    2015
    Citation
    Li, Xu, Wang, Wenqi, Wang, Jiadong, et al.. "Proteomic analyses reveal distinct chromatin‐associated and soluble transcription factor complexes." Molecular Systems Biology, 11, (2015) http://dx.doi.org/10.15252/msb.20145504.
    Published Version
    http://dx.doi.org/10.15252/msb.20145504
    Abstract
    The current knowledge on how transcription factors (TFs), the ultimate targets and executors of cellular signalling pathways, are regulated by protein–protein interactions remains limited. Here, we performed proteomics analyses of soluble and chromatin‐associated complexes of 56 TFs, including the targets of many signalling pathways involved in development and cancer, and 37 members of the Forkhead box (FOX) TF family. Using tandem affinity purification followed by mass spectrometry (TAP/MS), we performed 214 purifications and identified 2,156 high‐confident protein–protein interactions. We found that most TFs form very distinct protein complexes on and off chromatin. Using this data set, we categorized the transcription‐related or unrelated regulators for general or specific TFs. Our study offers a valuable resource of protein–protein interaction networks for a large number of TFs and underscores the general principle that TFs form distinct location‐specific protein complexes that are associated with the different regulation and diverse functions of these Tfs.
    Keyword
    orkhead box; mass spectrometry; protein-protein interaction; transcriptional factor
    Type
    Journal article
    Citable link to this page
    http://hdl.handle.net/1911/79056
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    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892