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dc.contributor.authorWu, Hui
Tuli, Leepika
Bennett, George N.
San, Ka-Yiu
dc.date.accessioned 2015-02-11T15:45:10Z
dc.date.available 2015-02-11T15:45:10Z
dc.date.issued 2015
dc.identifier.citation Wu, Hui, Tuli, Leepika, Bennett, George N., et al.. "Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli." Metabolic Engineering, 28, (2015) Elsevier: 159-168. http://dx.doi.org/10.1016/j.ymben.2015.01.002.
dc.identifier.urihttps://hdl.handle.net/1911/79028
dc.description.abstract A novel strategy to finely control a large metabolic flux by using a “metabolic transistor” approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a large effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The “metabolic transistor” strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. coli strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle Metabolic Engineering
dc.subject.keywordEscherichia coli
metabolic transistor
electron transfer chain
quinone
lactate
oxygen
dc.citation.volumeNumber 28
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.ymben.2015.01.002
dc.identifier.pmcid PMC4355220
dc.identifier.pmid 25596510
dc.identifier.grantID NIH GM090152 (National Institutes of Health)
dc.type.publication post-print
dc.citation.firstpage 159
dc.citation.lastpage 168


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