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    Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli

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    Author
    Wu, Hui; Tuli, Leepika; Bennett, George N.; San, Ka-Yiu
    Date
    2015
    Abstract
    A novel strategy to finely control a large metabolic flux by using a “metabolic transistor” approach was established. In this approach a small change in the level or availability of an essential component for the process is controlled by adding a competitive reaction that affects a precursor or an intermediate in its biosynthetic pathway. The change of the basal level of the essential component, considered as a base current in a transistor, has a large effect on the flux through the major pathway. In this way, the fine-tuning of a large flux can be accomplished. The “metabolic transistor” strategy was applied to control electron transfer chain function by manipulation of the quinone synthesis pathway in Escherichia coli. The achievement of a theoretical yield of lactate production under aerobic conditions via this strategy upon manipulation of the biosynthetic pathway of the key participant, ubiquinone-8 (Q8), in an E. coli strain provides an in vivo, genetically tunable means to control the activity of the electron transfer chain and manipulate the production of reduced products while limiting consumption of oxygen to a defined amount.
    Citation
    Wu, Hui, Tuli, Leepika, Bennett, George N., et al.. "Metabolic transistor strategy for controlling electron transfer chain activity in Escherichia coli." Metabolic Engineering, 28, (2015) Elsevier: 159-168. http://dx.doi.org/10.1016/j.ymben.2015.01.002.
    Published Version
    http://dx.doi.org/10.1016/j.ymben.2015.01.002
    Keyword
    Escherichia coli; metabolic transistor; electron transfer chain; quinone; lactate; More... oxygen Less...
    Type
    Journal article
    Publisher
    Elsevier
    Citable link to this page
    https://hdl.handle.net/1911/79028
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map