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    The Atlastin C-terminal Tail is an Amphipathic Helix that Perturbs Bilayer Structure during Endoplasmic Reticulum Homotypic Fusion

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    Author
    Faust, Joseph E.; Desai, Tanvi; Verma, Avani; Ulengin, Idil; Sun, Tzu-Lin; More... Moss, Tyler J.; Betancourt, Miguel A.; Huang, Huey W.; Lee, Tina; McNew, James A. Less...
    Date
    2015
    Abstract
    Fusion of tubular membranes is required to form three-way junctions found in reticular subdomains of the endoplasmic reticulum (ER). The large GTPase Atlastin has recently been shown to drive ER membrane fusion and three-way junction formation. The mechanism of Atlastin-mediated membrane fusion is distinct from SNARE-mediated and many details remain unclear. In particular, the role of the amphipathic C-terminal tail of Atlastin is still unknown. We have found that a peptide corresponding to the Atlastin C-terminal tail binds to membranes as a parallel alpha helix, induces bilayer thinning, and increases acyl chain disorder. The function of the C-terminal tail is conserved in human Atlastin. Mutations in the C-terminal tail decrease fusion activity in vitro, but not GTPase activity, and impair Atlastin function in vivo. In the context of unstable lipid bilayers, the requirement for the C-terminal tail is abrogated. These data suggest that the C-terminal tail of Atlastin locally destabilizes bilayers to facilitate membrane fusion.
    Citation
    Faust, Joseph E., Desai, Tanvi, Verma, Avani, et al.. "The Atlastin C-terminal Tail is an Amphipathic Helix that Perturbs Bilayer Structure during Endoplasmic Reticulum Homotypic Fusion." The Journal of Biological Chemistry, (2015) American Society for Biochemistry and Molecular Biology: http://dx.doi.org/10.1074/jbc.M114.601823.
    Published Version
    http://dx.doi.org/10.1074/jbc.M114.601823
    Keyword
    cell compartmentalization; endoplasmic reticulum (ER); membrane fusion; fluorescence resonance energy transfer (FRET); phospholipid vesicle; More... neurodegeneration; membrane structure; GTPase Less...
    Type
    Journal article
    Publisher
    American Society for Biochemistry and Molecular Biology
    Citable link to this page
    https://hdl.handle.net/1911/78925
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the American Society for Biochemistry and Molecular Biology.
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    • Biochemistry and Cell Biology Publications [118]
    • Faculty Publications [4988]
    • Physics and Astronomy Publications [1771]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map