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dc.contributor.authorKothawala, Ahmed
Kilpatrick, Kiri
Novoa, Jose Andres
Segatori, Laura
dc.date.accessioned 2015-01-06T19:20:34Z
dc.date.available 2015-01-06T19:20:34Z
dc.date.issued 2012
dc.identifier.citation Kothawala, Ahmed, Kilpatrick, Kiri, Novoa, Jose Andres, et al.. "Quantitative Analysis of α-Synuclein Solubility in Living Cells Using Split GFP Complementation." PLoS ONE, 7, no. 8 (2012) Public Library of Science: e43505. http://dx.doi.org/10.1371/journal.pone.0043505.
dc.identifier.urihttps://hdl.handle.net/1911/78897
dc.description.abstract Presently incurable, Parkinson's disease (PD) is the most common neurodegenerative movement disorder and affects 1% of the population over 60 years of age. The hallmarks of PD pathogenesis are the loss of dopaminergic neurons in the substantia nigra pars compacta, and the occurrence of proteinaceous cytoplasmic inclusions (Lewy bodies) in surviving neurons. Lewy bodies are mainly composed of the pre-synaptic protein alpha-synuclein (αsyn), an intrinsically unstructured, misfolding-prone protein with high propensity to aggregate. Quantifying the pool of soluble αsyn and monitoring αsyn aggregation in living cells is fundamental to study the molecular mechanisms of αsyn-induced cytotoxicity and develop therapeutic strategies to prevent αsyn aggregation. In this study, we report the use of a split GFP complementation assay to quantify αsyn solubility. Particularly, we investigated a series of naturally occurring and rationally designed αsyn variants and showed that this method can be used to study how αsyn sequence specificity affects its solubility. Furthermore, we demonstrated the utility of this assay to explore the influence of the cellular folding network on αsyn solubility. The results presented underscore the utility of the split GFP assay to quantify αsyn solubility in living cells.
dc.language.iso eng
dc.publisher Public Library of Science
dc.rights This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.title Quantitative Analysis of α-Synuclein Solubility in Living Cells Using Split GFP Complementation
dc.type Journal article
dc.citation.journalTitle PLoS ONE
dc.citation.volumeNumber 7
dc.citation.issueNumber 8
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0043505
dc.identifier.pmcid PMC3425482
dc.identifier.pmid 22927976
dc.type.publication publisher version
dc.citation.firstpage e43505


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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.