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dc.contributor.authorMeretoja, Ville V.
Dahlin, Rebecca L.
Kasper, F. Kurtis
Mikos, Antonios G.
dc.date.accessioned 2015-01-06T15:49:12Z
dc.date.available 2015-01-06T15:49:12Z
dc.date.issued 2012
dc.identifier.citation Meretoja, Ville V., Dahlin, Rebecca L., Kasper, F. Kurtis, et al.. "Enhanced chondrogenesis in co-cultures with articular chondrocytes and mesenchymal stem cells." Biomaterials, 33, no. 27 (2012) Elsevier: 6362-6369. http://dx.doi.org/10.1016/j.biomaterials.2012.05.042.
dc.identifier.urihttps://hdl.handle.net/1911/78886
dc.description.abstract In this work, articular chondrocytes (ACs) and mesenchymal stem cells (MSCs) with 1:1 and 1:3 cell ratios were co-cultured in order to evaluate if a majority of primary ACs can be replaced with MSCs without detrimental effects on in vitro chondrogenesis. We further used a xenogeneic culture model to study if such co-cultures can result in redifferentiation of passaged ACs. Cells were cultured in porous scaffolds for four weeks and their cellularity, cartilage-like matrix formation and chondrogenic gene expression levels (collagen I and II, aggrecan) were measured. Constructs with primary bovine ACs had ∼1.6 and 5.5 times higher final DNA and glycosaminoglycan contents, respectively, in comparison to those with culture expanded chondrocytes or MSCs harvested from the same animals. Equally robust chondrogenesis was also observed in co-cultures, even when up to 75% of primary ACs were initially replaced with MSCs. Furthermore, species-specific RT-PCR analysis indicated a gradual loss of MSCs in bovine-rabbit co-cultures. Finally, co-cultures using primary and culture expanded ACs resulted in similar outcomes. We conclude that the most promising cell source for cartilage engineering was the co-cultures, as the trophic effect of MSCs may highly increase the chondrogenic potential of ACs thus diminishing the problems with primary chondrocyte harvest and expansion.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Enhanced chondrogenesis in co-cultures with articular chondrocytes and mesenchymal stem cells
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle Biomaterials
dc.subject.keywordcartilage tissue engineering
chondrocyte
co-culture
gene expression
mesenchcymal stem cell
dc.citation.volumeNumber 33
dc.citation.issueNumber 27
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.biomaterials.2012.05.042
dc.identifier.pmcid PMC3392514
dc.identifier.pmid 22695067
dc.identifier.grantID R01 AR057083 (National Institutes of Health)
dc.type.publication post-print
dc.citation.firstpage 6362
dc.citation.lastpage 6369


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