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dc.contributor.authorMountziaris, Paschalia M.
Tzouanas, Stephanie N.
Sing, David C.
Kramer, Phillip R.
Kasper, F. Kurtis
Mikos, Antonios G.
dc.date.accessioned 2015-01-06T15:49:12Z
dc.date.available 2015-01-06T15:49:12Z
dc.date.issued 2012
dc.identifier.citation Mountziaris, Paschalia M., Tzouanas, Stephanie N., Sing, David C., et al.. "Intra-articular controlled release of anti-inflammatory siRNA with biodegradable polymer microparticles ameliorates temporomandibular joint inflammation." Acta Biomaterialia, 8, no. 10 (2012) Elsevier: 3552-3560. http://dx.doi.org/10.1016/j.actbio.2012.06.031.
dc.identifier.urihttps://hdl.handle.net/1911/78885
dc.description.abstract We investigated the in vivo therapeutic efficacy of an intra-articular controlled release system consisting of biodegradable poly(dl-lactic-co-glycolic acid) (PLGA) microparticles (MPs) encapsulating anti-inflammatory small interfering RNA (siRNA), together with branched poly(ethylenimine) (PEI) as a transfecting agent, in a rat model of painful temporomandibular joint (TMJ) inflammation. The in vivo effects of PLGA MP dose and siRNA-PEI polyplex delivery were examined via non-invasive meal pattern analysis and by quantifying the protein level of the siRNA target as well as of several downstream inflammatory cytokines. Controlled release of siRNA-PEI from PLGA MPs significantly reduced inflammation-induced changes in meal patterns compared to untreated rats with inflamed TMJs. These changes correlated to decreases in tissue-level protein expression of the siRNA target to 20-50% of the amount present in the corresponding control groups. Similar reductions were also observed in the expression of downstream inflammatory cytokines, e.g. interleukin-6, whose tissue levels in the siRNAヨPEI PLGA MP groups were 50% of the values for the corresponding controls. This intra-articular sustained release system has significant implications for the treatment of severe TMJ pain, and also has the potential to be readily adapted and applied to mitigate painful, chronic inflammation in a variety of conditions.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Intra-articular controlled release of anti-inflammatory siRNA with biodegradable polymer microparticles ameliorates temporomandibular joint inflammation
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle Acta Biomaterialia
dc.subject.keywordcontrolled drug release
drug delivery
microsphere
inflammation
immunomodulation
dc.citation.volumeNumber 8
dc.citation.issueNumber 10
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.actbio.2012.06.031
dc.identifier.pmcid PMC3429637
dc.identifier.pmid 22750740
dc.identifier.grantID R01 DE15164 (National Institutes of Health)
dc.identifier.grantID 5 T32 GM008362-19 (National Institutes of Health)
dc.type.publication post-print
dc.citation.firstpage 3552
dc.citation.lastpage 3560


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