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dc.contributor.authorPok, Seokwon
Myers, Jackson D.
Madihally, Sundararajan V.
Jacot, Jeffrey G.
dc.date.accessioned 2015-01-05T20:03:20Z
dc.date.available 2015-01-05T20:03:20Z
dc.date.issued 2013
dc.identifier.citation Pok, Seokwon, Myers, Jackson D., Madihally, Sundararajan V., et al.. "A multilayered scaffold of a chitosan and gelatin hydrogel supported by a PCL core for cardiac tissue engineering." Acta Biomaterialia, 9, no. 3 (2013) Elsevier: 5630-5642. http://dx.doi.org/10.1016/j.actbio.2012.10.032.
dc.identifier.urihttps://hdl.handle.net/1911/78851
dc.description.abstract A three-dimensional scaffold composed of self-assembled polycaprolactone (PCL) sandwiched in a gelatin–chitosan hydrogel was developed for use as a biodegradable patch with a potential for surgical reconstruction of congenital heart defects. The PCL core provides surgical handling, suturability and high initial tensile strength, while the gelatin–chitosan scaffold allows for cell attachment, with pore size and mechanical properties conducive to cardiomyocyte migration and function. The ultimate tensile stress of the PCL core, made from blends of 10, 46 and 80 kDa (Mn) PCL, was controllable in the range of 2–4 MPa, with lower average molecular weight PCL blends correlating with lower tensile stress. Blends with lower molecular weight PCL also had faster degradation (controllable from 0% to 7% weight loss in saline over 30 days) and larger pores. PCL scaffolds supporting a gelatin–chitosan emulsion gel showed no significant alteration in tensile stress, strain or tensile modulus. However, the compressive modulus of the composite tissue was similar to that of native tissue (∼15 kPa for 50% gelatin and 50% chitosan). Electron microscopy revealed that the gelatin–chitosan gel had a three-dimensional porous structure, with a mean pore diameter of ∼80 μm, showed migration of neonatal rat ventricular myocytes (NRVM), maintained NRVM viability for over 7 days, and resulted in spontaneously beating scaffolds. This multi-layered scaffold has sufficient tensile strength and surgical handling for use as a cardiac patch, while allowing migration or pre-loading of cardiac cells in a biomimetic environment to allow for eventual degradation of the patch and incorporation into native tissue.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title A multilayered scaffold of a chitosan and gelatin hydrogel supported by a PCL core for cardiac tissue engineering
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle Acta Biomaterialia
dc.subject.keywordcardiac patch
RVOT
hydrogel
gelatin
chitosan
polycaprolactone
myocardium
dc.citation.volumeNumber 9
dc.citation.issueNumber 3
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.actbio.2012.10.032
dc.identifier.pmcid PMC3562398
dc.identifier.pmid 23128158
dc.identifier.grantID 1R21HL110330 (National Institutes of Health)
dc.type.publication post-print
dc.citation.firstpage 5630
dc.citation.lastpage 5642


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