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    A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations

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    Author
    Spurlin, James III; Lwigale, Peter
    Date
    2013
    Abstract
    Background: During early development, avian embryos are easily accessible in ovo for transplantations and experimental perturbations. However, these qualities of the avian embryonic model rapidly wane shortly after embryonic day (E)4 when the embryo is obscured by extraembryonic membranes, making it difficult to study developmental events that occur at later stages in vivo. Results: In this study, we describe a multistep method that involves initially windowing eggs at E3, followed by dissecting away extraembryonic membranes at E5 to facilitate embryo accessibility in ovo until later stages of development. The majority of the embryos subjected to this technique remain exposed between E5 and E8, then become gradually displaced by the growing allantois from posterior to anterior regions. Conclusions: Exposed embryos are viable and compatible with embryological and modern developmental biology techniques including tissue grafting and ablation, gene manipulation by electroporation, and protein expression. This technique opens up new avenues for studying complex cellular interactions during organogenesis and can be further extrapolated to regeneration and stem cell studies.
    Citation
    Spurlin, James III and Lwigale, Peter. "A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations." Developmental Dynamics, 242, no. 2 (2013) Wiley: 148-154. http://dx.doi.org/10.1002/dvdy.23907.
    Published Version
    http://dx.doi.org/10.1002/dvdy.23907
    Keyword
    late-stage chick embryo; in ovo; extraembryonic membranes; dissection; cell injections
    Type
    Journal article
    Publisher
    Wiley
    Citable link to this page
    https://hdl.handle.net/1911/78759
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
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    • Biochemistry and Cell Biology Publications [118]
    • Faculty Publications [4990]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map