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dc.contributor.authorRohrman, Brittany A.
Richards-Kortum, Rebecca R.
dc.date.accessioned 2014-12-15T20:35:09Z
dc.date.available 2014-12-15T20:35:09Z
dc.date.issued 2012
dc.identifier.citation Rohrman, Brittany A. and Richards-Kortum, Rebecca R.. "A paper and plastic device for performing recombinase polymerase amplification of HIV DNA." Lab on a Chip, 12, no. 17 (2012) 3082-3088. http://dx.doi.org/10.1039/C2LC40423K.
dc.identifier.urihttps://hdl.handle.net/1911/78755
dc.description.abstract Despite the importance of early diagnosis and treatment of HIV, only a small fraction of HIV-exposed infants in low- and middle-income countries are tested for the disease. The gold standard for early infant diagnosis, DNA PCR, requires resources that are unavailable in poor settings, and no point-of-care HIV DNA test is currently available. We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA. The device is inexpensive, small, light-weight, and easy to assemble. The device stores lyophilized enzymes, facilitates mixing of reaction components, and supports recombinase polymerase amplification in five steps of operation. Using commercially available lateral flow strips as a detection method, we demonstrate the ability of our device to amplify 10 copies of HIV DNA to detectable levels in 15 minutes. Our results suggest that our device, which is designed to be used after DNA extraction from dried-blood spots, may serve in conjunction with lateral flow strips as part of a point-of-care HIV DNA test to be used in low resource settings.
dc.language.iso eng
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of Chemistry
dc.title A paper and plastic device for performing recombinase polymerase amplification of HIV DNA
dc.type Journal article
dc.citation.journalTitle Lab on a Chip
dc.citation.volumeNumber 12
dc.citation.issueNumber 17
dc.contributor.publisher Royal Society of Chemistry
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1039/C2LC40423K
dc.identifier.pmcid PMC3569001
dc.identifier.pmid 22733333
dc.type.publication post-print
dc.citation.firstpage 3082
dc.citation.lastpage 3088


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