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    A paper and plastic device for performing recombinase polymerase amplification of HIV DNA

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    Author
    Rohrman, Brittany A.; Richards-Kortum, Rebecca R.
    Date
    2012
    Abstract
    Despite the importance of early diagnosis and treatment of HIV, only a small fraction of HIV-exposed infants in low- and middle-income countries are tested for the disease. The gold standard for early infant diagnosis, DNA PCR, requires resources that are unavailable in poor settings, and no point-of-care HIV DNA test is currently available. We have developed a device constructed of layers of paper, glass fiber, and plastic that is capable of performing isothermal, enzymatic amplification of HIV DNA. The device is inexpensive, small, light-weight, and easy to assemble. The device stores lyophilized enzymes, facilitates mixing of reaction components, and supports recombinase polymerase amplification in five steps of operation. Using commercially available lateral flow strips as a detection method, we demonstrate the ability of our device to amplify 10 copies of HIV DNA to detectable levels in 15 minutes. Our results suggest that our device, which is designed to be used after DNA extraction from dried-blood spots, may serve in conjunction with lateral flow strips as part of a point-of-care HIV DNA test to be used in low resource settings.
    Citation
    Rohrman, Brittany A. and Richards-Kortum, Rebecca R.. "A paper and plastic device for performing recombinase polymerase amplification of HIV DNA." Lab on a Chip, 12, no. 17 (2012) Royal Society of Chemistry: 3082-3088. http://dx.doi.org/10.1039/C2LC40423K.
    Published Version
    http://dx.doi.org/10.1039/C2LC40423K
    Type
    Journal article
    Publisher
    Royal Society of Chemistry
    Citable link to this page
    https://hdl.handle.net/1911/78755
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Royal Society of Chemistry
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    • Bioengineering Publications [684]
    • Faculty Publications [5504]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map