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dc.contributor.authorDobbs, Jessica
Krishnamurthy, Savitri
Kyrish, Matthew
Benveniste, Ana Paula
Yang, Wei
Richards-Kortum, Rebecca
dc.date.accessioned 2014-12-01T17:26:01Z
dc.date.available 2014-12-01T17:26:01Z
dc.date.issued 2014
dc.identifier.citation Dobbs, Jessica, Krishnamurthy, Savitri, Kyrish, Matthew, et al.. "Confocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsies." Breast Cancer Research and Treatment, (2014) Springer: http://dx.doi.org/10.1007/s10549-014-3182-5.
dc.identifier.urihttps://hdl.handle.net/1911/78536
dc.description.abstract Tissue sampling is a problematic issue for inflammatory breast carcinoma, and immediate evaluation following core needle biopsy is needed to evaluate specimen adequacy. We sought to determine if confocal fluorescence microscopy provides sufficient resolution to evaluate specimen adequacy by comparing invasive tumor cellularity estimated from standard histologic images to invasive tumor cellularity estimated from confocal images of breast core needle biopsy specimens. Grayscale confocal fluorescence images of breast core needle biopsy specimens were acquired following proflavine application. A breast-dedicated pathologist evaluated invasive tumor cellularity in histologic images with hematoxylin and eosin staining and in grayscale and false-colored confocal images of cores. Agreement between cellularity estimates was quantified using a kappa coefficient. 23 cores from 23 patients with suspected inflammatory breast carcinoma were imaged. Confocal images were acquired in an average of less than 2 min per core. Invasive tumor cellularity estimated from histologic and grayscale confocal images showed moderate agreement by kappa coefficient: κ = 0.48 ± 0.09 (p < 0.001). Grayscale confocal images require less than 2 min for acquisition and allow for evaluation of invasive tumor cellularity in breast core needle biopsy specimens with moderate agreement to histologic images. We show that confocal fluorescence microscopy can be performed immediately following specimen acquisition and could indicate the need for additional biopsies at the initial visit.
dc.language.iso eng
dc.publisher Springer
dc.rights This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/
dc.title Confocal fluorescence microscopy for rapid evaluation of invasive tumor cellularity of inflammatory breast carcinoma core needle biopsies
dc.type Journal article
dc.contributor.funder U.S. Department of Defense
dc.contributor.funder Susan G. Komen for the Cure
dc.contributor.funder Morgan Welch Inflammatory Breast Cancer Research Program
dc.citation.journalTitle Breast Cancer Research and Treatment
dc.subject.keywordinflammatory breast cancer
core needle biopsy
confocal fluorescence microscopy
tissue sampling
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1007/s10549-014-3182-5
dc.identifier.pmcid PMC4298669
dc.identifier.pmid 25417171
dc.identifier.grantID Era of Hope Award W81XWH-09-1-0410 (U.S. Department of Defense)
dc.identifier.grantID KG091020 (Susan G. Komen for the Cure)
dc.type.publication publisher version


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This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
Except where otherwise noted, this item's license is described as This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.