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dc.contributor.authorGao, Yang
Connell, Jennifer Petsche
Wadhwa, Lalita
Ruano, Rodrigo
Jacot, Jeffrey G.
dc.date.accessioned 2014-11-21T21:26:58Z
dc.date.available 2014-11-21T21:26:58Z
dc.date.issued 2014
dc.identifier.citation Gao, Yang, Connell, Jennifer Petsche, Wadhwa, Lalita, et al.. "Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells." Annals of Biomedical Engineering, 42, no. 12 (2014) 2490-2500. http://dx.doi.org/10.1007/s10439-014-1114-5.
dc.identifier.urihttps://hdl.handle.net/1911/78490
dc.description.abstract Amniotic fluid-derived stem cells (AFSC) have been shown to be broadly multipotent and non-tumorogenic. Previous studies of direct mixing of AFSC and neonatal rat ventricle myocytes indicated evidence of AFSC cardiogenesis. In this study, we examined human AFSC cardiogenic potential in indirect co-culture with human cardiac cells in conditions that eliminated the possibility of cell fusion. Human AFSC in contact with human cardiac cells showed expression of cardiac troponin T (cTnT) in immunohistochemistry, and no evidence of cell fusion were found through fluorescent in situ hybridization. When indirectly co-cultured with cardiac cells, human AFSC in contact with cardiac cells across a thin porous membrane showed a statistically significant increase in cTnT expression compared to non-contact conditions but lacked upregulation of calcium modulating proteins and did not have functional or morphological characteristics of mature cardiomyocytes. This suggests that contact is a necessary but not sufficient condition for AFSC cardiac differentiation in co-culture with cardiac cells.
dc.language.iso eng
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.
dc.title Amniotic Fluid-Derived Stem Cells Demonstrated Cardiogenic Potential in Indirect Co-culture with Human Cardiac Cells
dc.type Journal article
dc.contributor.funder American Heart Association
dc.citation.journalTitle Annals of Biomedical Engineering
dc.subject.keywordcardiomyocytes
cardiac progenitor cells
right ventricular out flow tract
pediatric tissue engineering
congenital heart defects
dc.citation.volumeNumber 42
dc.citation.issueNumber 12
dc.contributor.publisher Springer
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1007/s10439-014-1114-5
dc.identifier.pmcid PMC4241123
dc.identifier.pmid 25266932
dc.identifier.grantID Beginning Grant-in-Aid (American Heart Association)
dc.type.publication post-print
dc.citation.firstpage 2490
dc.citation.lastpage 2500


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