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dc.contributor.authorCordray, Michael S.
Amdahl, Matthew
Richards-Kortum, Rebecca R.
dc.date.accessioned 2014-10-29T18:15:02Z
dc.date.available 2014-10-29T18:15:02Z
dc.date.issued 2012
dc.identifier.citation Cordray, Michael S., Amdahl, Matthew and Richards-Kortum, Rebecca R.. "Gold Nanoparticle Aggregation for Quantification of Oligonucleotides: Optimization and Increased Dynamic Range." Analytical Biochemistry, 431, no. 2 (2012) Elsevier: 99-105. http://dx.doi.org/10.1016/j.ab.2012.09.013.
dc.identifier.urihttps://hdl.handle.net/1911/77666
dc.description.abstract A variety of assays have been proposed to detect small quantities of nucleic acids at the point-of-care. One approach relies on target-induced aggregation of gold nanoparticles functionalized with oligonucleotide sequences complementary to adjacent regions on the targeted sequence. In the presence of the target sequence, the gold nanoparticles aggregate, producing an easily detectable shift in the optical scattering properties of the solution. The major limitations of this assay are that it requires heating, and that long incubation times are required to produce a result. This study aims to optimize the assay conditions and optical readout, with the goals of eliminating the need for heating and reducing the time to result without sacrificing sensitivity or dynamic range. By optimizing assay conditions and measuring the spectrum of scattered light at the endpoint of incubation, we find that the assay is capable of producing quantifiable results at room temperature in 30 minutes with a linear dynamic range spanning from 150 amoles to 15 fmoles of target. If changes in light scattering are measured dynamically during the incubation process, the linear range can be expanded 2-fold, spanning 50 amoles to 500 fmoles, while decreasing the time to result down to 10 minutes.
dc.language.iso eng
dc.publisher Elsevier
dc.rights Open access under CC BY-NC-ND license .
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/3.0/
dc.title Gold Nanoparticle Aggregation for Quantification of Oligonucleotides: Optimization and Increased Dynamic Range
dc.type Journal article
dc.citation.journalTitle Analytical Biochemistry
dc.subject.keywordgold nanoparticles
oligonucleotides
optical scattering
quantification
dc.citation.volumeNumber 431
dc.citation.issueNumber 2
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.ab.2012.09.013
dc.identifier.pmcid PMC3496753
dc.identifier.pmid 23000603
dc.type.publication publisher version
dc.citation.firstpage 99
dc.citation.lastpage 105


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