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    IL-1β induces p62/SQSTM1 and represses androgen receptor expression in prostate cancer cells

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    Chang, M.A.; Patel, V.; Gwede, M.; Morgado, M.; Tomasevich, K.; More... Fong, E.L.; Farach-Carson, M.C.; Delk, N.A. Less...
    Date
    2014
    Abstract
    Chronic inflammation is associated with advanced prostate cancer (PCa), although the mechanisms governing inflammation-mediated PCa progression are not fully understood. PCa progresses to an androgen independent phenotype that is incurable. We previously showed that androgen independent, androgen receptor negative (AR−) PCa cell lines have high p62/SQSTM1 levels required for cell survival. We also showed that factors in the HS-5 bone marrow stromal cell (BMSC) conditioned medium can upregulate p62 in AR+ PCa cell lines, leading us to investigate AR expression under those growth conditions. In this paper, mRNA, protein, and subcellular analyses reveal that HS-5 BMSC conditioned medium represses AR mRNA, protein, and nuclear accumulation in the C4-2 PCa cell line. Using published gene expression data, we identify the inflammatory cytokine, IL-1β, as a candidate BMSC paracrine factor to regulate AR expression and find that IL-1β is sufficient to both repress AR and upregulate p62 in multiple PCa cell lines. Immunostaining demonstrates that, while the C4-2 population shows a primarily homogeneous response to factors in HS-5 BMSC conditioned medium, IL-1β elicits a strikingly heterogeneous response; suggesting that there are other regulatory factors in the conditioned medium. Finally, while we observe concomitant AR loss and p62 upregulation in IL-1β-treated C4-2 cells, silencing of AR or p62 suggests that IL-1β regulates their protein accumulation through independent pathways. Taken together, these in vitro results suggest that IL-1β can drive PCa progression in an inflammatory microenvironment through AR repression and p62 induction to promote the development and survival of androgen independent Pca.
    Citation
    Chang, M.A., Patel, V., Gwede, M., et al.. "IL-1β induces p62/SQSTM1 and represses androgen receptor expression in prostate cancer cells." Journal of Cellular Biochemistry, 115, no. 12 (2014) John Wiley & Sons, Inc.: 2188-2197. http://dx.doi.org/10.1002/jcb.24897.
    Published Version
    http://dx.doi.org/10.1002/jcb.24897
    Keyword
    Interleukin-1β; p62/Sequestome-1; androgen receptor; prostate cancer; bone marrow stromal cells; More... inflammation Less...
    Type
    Journal article
    Publisher
    John Wiley & Sons, Inc.
    Citable link to this page
    https://hdl.handle.net/1911/77498
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Wiley.
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    • Biochemistry and Cell Biology Publications [118]
    • Faculty Publications [4978]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map