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dc.contributor.authorUppulury, Karthik
Efremov, Artem K.
Driver, Jonathan W.
Jamison, D. Kenneth
Diehl, Michael R.
Kolomeisky, Anatoly B.
dc.date.accessioned 2014-10-08T21:25:36Z
dc.date.available 2014-10-08T21:25:36Z
dc.date.issued 2013
dc.identifier.citation Uppulury, Karthik, Efremov, Artem K., Driver, Jonathan W., et al.. "Analysis of Cooperative Behavior in Multiple Kinesins Motor Protein Transport by Varying Structural and Chemical Properties." Cellular and Molecular Bioengineering, 6, no. 1 (2013) Springer: 38-47. http://dx.doi.org/10.1007/s12195-012-0260-9.
dc.identifier.urihttps://hdl.handle.net/1911/77454
dc.description.abstract Intracellular transport is a fundamental biological process during which cellular materials are driven by enzymatic molecules called motor proteins. Recent optical trapping experiments and theoretical analysis have uncovered many features of cargo transport by multiple kinesin motor protein molecules under applied loads. These studies suggest that kinesins cooperate negatively under typical transport conditions, although some productive cooperation could be achieved under higher applied loads. However, the microscopic origins of this complex behavior are still not well understood. Using a discrete-state stochastic approach we analyze factors that affect the cooperativity among kinesin motors during cargo transport. Kinesin cooperation is shown to be largely unaffected by the structural and mechanical parameters of a multiple motor complex connected to a cargo, but much more sensitive to biochemical parameters affecting motor-filament affinities. While such behavior suggests the net negative cooperative responses of kinesins will persist across a relatively wide range of cargo types, it is also shown that the rates with which cargo velocities relax in time upon force perturbations are influenced by structural factors that affect the free energies of and load distributions within a multiple kinesin complex. The implications of these later results on transport phenomena where loads change temporally, as in the case of bidirectional transport, are discussed.
dc.language.iso eng
dc.publisher Springer
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Springer.
dc.title Analysis of Cooperative Behavior in Multiple Kinesins Motor Protein Transport by Varying Structural and Chemical Properties
dc.type Journal article
dc.contributor.funder National Science Foundation
dc.contributor.funder National Institutes of Health
dc.contributor.funder Welch Foundation
dc.citation.journalTitle Cellular and Molecular Bioengineering
dc.subject.keywordintracellular transport
kinesin
cooperativity
dc.citation.volumeNumber 6
dc.citation.issueNumber 1
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1007/s12195-012-0260-9
dc.identifier.pmcid PMC3904397
dc.identifier.pmid 24489614
dc.identifier.grantID MCB-0643832 (National Science Foundation)
dc.identifier.grantID 1R01GM094489-01 (National Institutes of Health)
dc.identifier.grantID C-1559 (Welch Foundation)
dc.identifier.grantID C-1625 (Welch Foundation)
dc.type.publication post-print
dc.citation.firstpage 38
dc.citation.lastpage 47


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