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dc.contributor.authorCooper, David
Uhm, Heui
Tauzin, Lawrence J.
Poddar, Nitesh
Landes, Christy F.
dc.date.accessioned 2014-10-06T17:18:01Z
dc.date.available 2014-10-06T17:18:01Z
dc.date.issued 2013
dc.identifier.citation Cooper, David, Uhm, Heui, Tauzin, Lawrence J., et al.. "Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems." ChemBioChem, 14, no. 9 (2013) John Wiley & Sons, Inc.: 1075-1080. http://dx.doi.org/10.1002/cbic.201300030.
dc.identifier.urihttps://hdl.handle.net/1911/77399
dc.description.abstract We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes
dc.language.iso eng
dc.publisher John Wiley & Sons, Inc.
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrightedby John Wiley & Sons Ltd.
dc.title Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems
dc.type Journal article
dc.contributor.funder Welch Foundation
dc.contributor.funder National Science Foundation
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle ChemBioChem
dc.subject.keywordDNA
fluorophore
FRET
photobleach
single-molecule studies
dc.citation.volumeNumber 14
dc.citation.issueNumber 9
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1002/cbic.201300030
dc.identifier.pmcid PMC3871170
dc.identifier.pmid 23733413
dc.identifier.grantID C-1787 (Welch Foundation)
dc.identifier.grantID CBET-1133965 (National Science Foundation)
dc.identifier.grantID CHE-1151647 (National Science Foundation)
dc.identifier.grantID GM94246-01A1 (National Institutes of Health)
dc.type.publication post-print
dc.citation.firstpage 1075
dc.citation.lastpage 1080


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