Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems

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Author
Cooper, David
Uhm, Heui
Tauzin, Lawrence J.
Poddar, Nitesh
Landes, Christy F.
Date
2013
Citation
Cooper, David, Uhm, Heui, Tauzin, Lawrence J., et al.. "Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems." ChemBioChem, 14, no. 9 (2013) John Wiley & Sons, Inc.: 1075-1080. http://dx.doi.org/10.1002/cbic.201300030.
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Abstract
We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes
Keyword
DNA; fluorophore; FRET; photobleach; single-molecule studies
Type
Journal article
Publisher
John Wiley & Sons, Inc.
Citable link to this page
https://hdl.handle.net/1911/77399
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