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    Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems

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    Author
    Cooper, David; Uhm, Heui; Tauzin, Lawrence J.; Poddar, Nitesh; Landes, Christy F.
    Date
    2013
    Abstract
    We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes
    Citation
    Cooper, David, Uhm, Heui, Tauzin, Lawrence J., et al.. "Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems." ChemBioChem, 14, no. 9 (2013) John Wiley & Sons, Inc.: 1075-1080. http://dx.doi.org/10.1002/cbic.201300030.
    Published Version
    http://dx.doi.org/10.1002/cbic.201300030
    Keyword
    DNA; fluorophore; FRET; photobleach; single-molecule studies
    Type
    Journal article
    Publisher
    John Wiley & Sons, Inc.
    Citable link to this page
    https://hdl.handle.net/1911/77399
    Rights
    This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrightedby John Wiley & Sons Ltd.
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    • Chemistry Publications [636]
    • ECE Publications [1442]
    • Faculty Publications [4978]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map