Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems

Cooper, David; Uhm, Heui; Tauzin, Lawrence J.; Poddar, Nitesh; Landes, Christy F.

doi:http://dx.doi.org/10.1002/cbic.201300030

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Author

Cooper, David

Uhm, Heui

Tauzin, Lawrence J.

Poddar, Nitesh

Landes, Christy F.

Date

2013

Citation

Cooper, David, Uhm, Heui, Tauzin, Lawrence J., et al. and . "Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems." ChemBioChem, 14, no. 9 (2013) 1075-1080. http://dx.doi.org/10.1002/cbic.201300030.

Published Version

http://dx.doi.org/10.1002/cbic.201300030

Abstract

We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes

Keyword

DNA; fluorophore; FRET; photobleach; single-molecule studies

Type

Journal article

Citable link to this page

http://hdl.handle.net/1911/77399

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