Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems

Cooper, David; Uhm, Heui; Tauzin, Lawrence J.; Poddar, Nitesh; Landes, Christy F.

doi:http://dx.doi.org/10.1002/cbic.201300030

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Author

Cooper, David

Uhm, Heui

Tauzin, Lawrence J.

Poddar, Nitesh

Landes, Christy F.

Date

2013

Citation

Cooper, David, Uhm, Heui, Tauzin, Lawrence J., et al.. "Photobleaching Lifetimes of Cyanine Fluorophores Used for Single Molecule Förster Resonance Energy Transfer in the Presence of Various Photoprotection Systems." ChemBioChem, 14, no. 9 (2013) 1075-1080. http://dx.doi.org/10.1002/cbic.201300030.

Published Version

http://dx.doi.org/10.1002/cbic.201300030

Abstract

We investigated various photoprotection system combinations to find the combination that optimally extended the photobleach lifetime of a Cy3/Cy5 smFRET pair attached to a DNA hairpin in a single-molecule environment. We found that the glucose/glucose oxygen-scavenging solution in combination with redox-based photostabilization solutions yielded the longest average photobleaching lifetimes

Keyword

DNA; fluorophore; FRET; photobleach; single-molecule studies

Type

Journal article

Citable link to this page

http://hdl.handle.net/1911/77399

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