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dc.contributor.advisor Alvarez, Pedro J.
dc.creatorVega, Leticia
dc.date.accessioned 2014-09-30T20:59:53Z
dc.date.available 2014-09-30T20:59:53Z
dc.date.created 2013-05
dc.date.issued 2013-04-18
dc.date.submitted May 2013
dc.identifier.citation Vega, Leticia. "The Impact of Nickel on Lux I/LuxR-Type Quorum Sensing and Biofilm Formation of Environmental Proteobacterial Species." (2013) Diss., Rice University. https://hdl.handle.net/1911/77344.
dc.identifier.urihttps://hdl.handle.net/1911/77344
dc.description.abstract The ability to control the establishment and development of a biofilm is important in environmental engineering where biofilms are used to recycle wastes and remove toxins from the environment, or conversely, reduce the life of infrastructure due to corrosion. This thesis reports on the metal nickel and its influence on biofilm formation. Using a combination of bench top and molecular methods, I demonstrate that nickel ions decrease the expression of N-acyl homoserine lactone synthase and transcriptional activator genes and subsequently, biofilm formation in Burkholderia multivorans ATCC 17616 without affecting overall viability. I further demonstrate that this phenomenon is not limited to nickel and/or B. multivorans. I have identified an additional heavy metal, cadmium that down regulates the expression of homoserine lactone genes and subsequently decreases surface colonization. Traits such as twitching motility, elastase and pyocyanin production, which are influenced by quorum sensing mechanisms in Burkholderia and Pseudomonas, are also inhibited by nickel and cadmium ions.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectBiofilm
Quorum sensing
N-acyl homoserine lactone
Nickel
Cadmium
dc.title The Impact of Nickel on Lux I/LuxR-Type Quorum Sensing and Biofilm Formation of Environmental Proteobacterial Species
dc.contributor.committeeMember Tomson, Mason B.
dc.contributor.committeeMember Silberg, Jonathan J.
dc.contributor.committeeMember Pyle, Barry
dc.contributor.committeeMember McLean, Robert
dc.date.updated 2014-09-30T20:59:53Z
dc.identifier.slug 123456789/ETD-2013-05-544
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Civil and Environmental Engineering
thesis.degree.discipline Engineering
thesis.degree.grantor Rice University
thesis.degree.level Doctoral
thesis.degree.name Doctor of Philosophy


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