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dc.contributor.authorMiller, Jordan S.
Stevens, Kelly R.
Yang, Michael T.
Baker, Brendon M.
Nguyen, Duc-Huy T.
Cohen, Daniel M.
Toro, Esteban
Chen, Alice A.
Galie, Peter A.
Yu, Xiang
Chaturvedi, Ritika
Bhatia, Sangeeta N.
Chen, Christopher S.
dc.date.accessioned 2014-09-22T20:23:28Z
dc.date.available 2014-09-22T20:23:28Z
dc.date.issued 2012
dc.identifier.citation Miller, Jordan S., Stevens, Kelly R., Yang, Michael T., et al.. "Rapid casting of patterned vascular networks for perfusable engineered 3D tissues." Nature Materials, 11, no. 9 (2012) Nature Publishing Group: 768-774. http://dx.doi.org/10.1038/nmat3357.
dc.identifier.urihttps://hdl.handle.net/1911/77220
dc.description.abstract In the absence of perfusable vascular networks, three-dimensional (3D) engineered tissues densely populated with cells quickly develop a necrotic core [1]. Yet the lack of a general approach to rapidly construct such networks remains a major challenge for 3D tissue culture [2-4]. Here, we 3D printed rigid filament networks of carbohydrate glass, and used them as a cytocompatible sacrificial template in engineered tissues containing living cells to generate cylindrical networks which could be lined with endothelial cells and perfused with blood under high-pressure pulsatile flow. Because this simple vascular casting approach allows independent control of network geometry, endothelialization, and extravascular tissue, it is compatible with a wide variety of cell types, synthetic and natural extracellular matrices (ECMs), and crosslinking strategies. We also demonstrated that the perfused vascular channels sustained the metabolic function of primary rat hepatocytes in engineered tissue constructs that otherwise exhibited suppressed function in their core.
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Nature Publishing Group.
dc.title Rapid casting of patterned vascular networks for perfusable engineered 3D tissues
dc.type Journal article
dc.citation.journalTitle Nature Materials
dc.citation.volumeNumber 11
dc.citation.issueNumber 9
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1038/nmat3357
dc.identifier.pmcid PMC3586565
dc.identifier.pmid 22751181
dc.type.publication post-print
dc.citation.firstpage 768
dc.citation.lastpage 774


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