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dc.contributor.advisor Tao, Yizhi Jane
dc.creatorHarper, Justin
dc.date.accessioned 2014-09-11T16:28:21Z
dc.date.available 2014-09-11T16:28:21Z
dc.date.created 2013-12
dc.date.issued 2013-06-28
dc.date.submitted December 2013
dc.identifier.citation Harper, Justin. "The Induction of Infectivity in Human Astrovirus in Response to Capsid Proteolysis." (2013) Master’s Thesis, Rice University. https://hdl.handle.net/1911/77163.
dc.identifier.urihttps://hdl.handle.net/1911/77163
dc.description.abstract Astrovirus is a non-enveloped, T=3, positive-sense RNA virus that presents with self-limiting gastroenteritis; however, it has been additionally associated with serious presentations such as nephritis, hepatitis, and encephalitis, which is compounded by its propensity to engage in cross-species penetrations. Astrovirus undergoes a complex capsid maturation process mediated by host proteases in which an inert, immature capsid composed of VP90 is sequentially cleaved to yield a highly infectious particle composed of VP34 and VP27/VP25, which form the capsid shell and spikes, respectively. By overexpressing a VP9070-418 truncate in insect cells, we have demonstrated that the shell domain alone cannot support particle assembly, implying a crucial role for the dimeric contacts within the spike. Various monomeric, shell domain truncates (i.e. VP9071-252, VP9071-283, VP9071-313, and VP9071-415) have been successfully expressed and purified, but none yielded useful crystals, suggesting the structural context of the capsid lattice may be needed to stabilize their conformational flexibility.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectX-ray crystallography
Virology
Astrovirus
Infectivity
Structure
Assembly
Biophysics
Biochemistry
HAstV
Capsid
RNA virus
dc.title The Induction of Infectivity in Human Astrovirus in Response to Capsid Proteolysis
dc.contributor.committeeMember McNew, James A.
dc.contributor.committeeMember Stewart, Charles R.
dc.date.updated 2014-09-11T16:28:21Z
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biochemistry and Cell Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Arts


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