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dc.contributor.authorKima, Kyobum
Lama, Johnny
Lua, Steven
Spicer, Patrick P.
Lueckgena, Aline
Yasuhiko, Tabata
Wong, Mark E.
Jansen, John A.
Mikos, Antonios G.
Kasper, F. Kurtis
dc.date.accessioned 2014-03-06T18:54:05Z
dc.date.available 2014-03-06T18:54:05Z
dc.date.issued 2014
dc.identifier.citation Kima, Kyobum, Lama, Johnny, Lua, Steven, et al.. "Osteochondral Tissue Regeneration using a Bilayered Composite Hydrogel with Modulating Dual Growth Factor Release Kinetics in a Rabbit Model." Journal of Controlled Release, 168, no. 2 (2014) Elsevier: 166-178. http://dx.doi.org/10.1016/j.jconrel.2013.03.013.
dc.identifier.urihttps://hdl.handle.net/1911/75554
dc.description.abstract Biodegradable oligo(poly(ethylene glycol) fumarate) (OPF) composite hydrogels have been investigated for the delivery of growth factors (GFs) with the aid of gelatin microparticles (GMPs) and stem cell populations for osteochondral tissue regeneration. In this study, a bilayered OPF composite hydrogel that mimics the distinctive hierarchical structure of native osteochondral tissue was utilized to investigate the effect of transforming growth factor-β3 (TGF-β3) with varying release kinetics and/or insulin-like growth factor-1 (IGF-1) on osteochondral tissue regeneration in a rabbit full-thickness osteochondral defect model. The four groups investigated included (i) a blank control (no GFs), (ii) GMP-loaded IGF-1 alone, (iii) GMP-loaded IGF-1 and gel-loaded TGF-β3, and (iv) GMP-loaded IGF-1 and GMP-loaded TGF-β3 in OPF composite hydrogels. The results of an in vitro release study demonstrated that TGF-β3 release kinetics could be modulated by the GF incorporation method. At 12 weeks post-implantation, the quality of tissue repair in both chondral and subchondral layers was analyzed based on quantitative histological scoring. All groups incorporating GFs resulted in a significant improvement in cartilage morphology compared to the control. Single delivery of IGF-1 showed higher scores in subchondral bone morphology as well as chondrocyte and glycosaminoglycan amount in adjacent cartilage tissue when compared to a dual delivery of IGF-1 and TGF-β3, independent of the TGF-?3 release kinetics. The results suggest that although the dual delivery of TGF-β3 and IGF-1 may not synergistically enhance the quality of engineered tissue, the delivery of IGF-1 alone from bilayered composite hydrogels positively affects osteochondral tissue repair and holds promise for osteochondral tissue engineering applications.
dc.language.iso eng
dc.publisher Elsevier
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by Elsevier.
dc.title Osteochondral Tissue Regeneration using a Bilayered Composite Hydrogel with Modulating Dual Growth Factor Release Kinetics in a Rabbit Model
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.citation.journalTitle Journal of Controlled Release
dc.subject.keywordhydrogel
osteochondral defect
transforming growth factor-β3
insulin-like growth factor-1
dc.citation.volumeNumber 168
dc.citation.issueNumber 2
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1016/j.jconrel.2013.03.013
dc.identifier.pmcid PMC3661728
dc.identifier.pmid 23541928
dc.identifier.grantID R01-AR048756 (National Institutes of Health)
dc.type.publication post-print
dc.citation.firstpage 166
dc.citation.lastpage 178


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