|dc.contributor.author||Lim, Chin Tat
Afizah, Mohd Hassan
Chian, Kerm Sin
Mikos, Antonios G.
Hui, James Hoi Po
Lim, Chin Tat, Ren, Xiafei, Afizah, Mohd Hassan, et al.. "Repair of Osteochondral Defects with Rehydrated Freeze-Dried Oligo[Poly(Ethylene Glycol) Fumarate] Hydrogels Seeded with Bone Marrow Mesenchymal Stem Cells in a Porcine Model." Tissue Engineering: Part A, 19, no. 15-16 (2013) 1852-1861. http://dx.doi.org/10.1089/ten.tea.2012.0621.
Current surgical techniques for osteochondral injuries in young active patients are inadequate clinically. Novel
strategies in tissue engineering are continuously explored in this area. Despite numerous animal studies that have
shown encouraging results, very few large-scale clinical trials have been done to address this area of interest. To
facilitate the eventual translation from rabbit to human subjects, we have performed a study using bone marrowderived
mesenchymal stem cell (BMSC)ﾖoligo[poly(ethylene glycol) fumarate] (OPF) hydrogel scaffold in a porcine
model. Our objective was to analyze the morphology of BMSCs seeded into rehydrated freeze-dried OPF hydrogel
and in vivo gross morphological and histological outcome of defects implanted with the BMSCs-OPF scaffold in a
porcine model. The analyses were based on magnified histologic sections for different types of cartilage repair
tissues, the outcome of the subchondral bone, scaffold, and statistical assessment of neotissue-filling percentage,
cartilage phenotype, and Wakitani scores. The morphology of the BMSCs seeded into the rehydrated freeze-dried
OPF scaffold was observed 24 h after cell seeding, through the phase-contrast microscope. The three-dimensional
and cross-sectional structure of the fabrication was analyzed through confocal microscopy and histological
methods, respectively. The BMSCs remained viable and were condensed into many pellet-like cell masses with a
diameter ranging from 28.5 to 298.4 (113.5 - 47.9) mm in the OPF scaffold. In vivo osteochondral defect repair was
tested in 12 defects created in six 8-month-old Prestige World Genetics micropigs. The implantation of scaffold
alone was used for control. Gross morphological, histological, and statistical analyses were performed at 4 months
postoperatively. The scaffoldﾖMSC treatment led to 99% defect filling, with 84% hyaline-like cartilage at 4 months,
which was significantly ( p < 0.0001) more than the 54% neotissue filling and 39% hyaline-like cartilage obtained in
the scaffold-only group. The implantation of BMSCs in freeze-dried OPF hydrogel scaffold, which created a
conducive environment for cell infiltration and clustering, could fully repair chondral defects with hyaline-like
cartilage. This protocol provides a clinically feasible procedure for osteochondral defect treatment.
Repair of Osteochondral Defects with Rehydrated Freeze-Dried Oligo[Poly(Ethylene Glycol) Fumarate] Hydrogels Seeded with Bone Marrow Mesenchymal Stem Cells in a Porcine Model
Tissue Engineering: Part A
BioScience Research Collaborative
Mary Ann Liebert, Inc.