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dc.contributor.authorLim, Chin Tat
Ren, Xiafei
Afizah, Mohd Hassan
Tarigan-Panjaitan, Sari
Yang, Zheng
Wu, Yingnan
Chian, Kerm Sin
Mikos, Antonios G.
Hui, James Hoi Po
dc.date.accessioned 2013-12-20T19:41:34Z
dc.date.available 2013-12-20T19:41:34Z
dc.date.issued 2013
dc.identifier.citation Lim, Chin Tat, Ren, Xiafei, Afizah, Mohd Hassan, et al.. "Repair of Osteochondral Defects with Rehydrated Freeze-Dried Oligo[Poly(Ethylene Glycol) Fumarate] Hydrogels Seeded with Bone Marrow Mesenchymal Stem Cells in a Porcine Model." Tissue Engineering: Part A, 19, no. 15-16 (2013) 1852-1861. http://dx.doi.org/10.1089/ten.tea.2012.0621.
dc.identifier.urihttp://hdl.handle.net/1911/75303
dc.description.abstract Current surgical techniques for osteochondral injuries in young active patients are inadequate clinically. Novel strategies in tissue engineering are continuously explored in this area. Despite numerous animal studies that have shown encouraging results, very few large-scale clinical trials have been done to address this area of interest. To facilitate the eventual translation from rabbit to human subjects, we have performed a study using bone marrowderived mesenchymal stem cell (BMSC)ヨoligo[poly(ethylene glycol) fumarate] (OPF) hydrogel scaffold in a porcine model. Our objective was to analyze the morphology of BMSCs seeded into rehydrated freeze-dried OPF hydrogel and in vivo gross morphological and histological outcome of defects implanted with the BMSCs-OPF scaffold in a porcine model. The analyses were based on magnified histologic sections for different types of cartilage repair tissues, the outcome of the subchondral bone, scaffold, and statistical assessment of neotissue-filling percentage, cartilage phenotype, and Wakitani scores. The morphology of the BMSCs seeded into the rehydrated freeze-dried OPF scaffold was observed 24 h after cell seeding, through the phase-contrast microscope. The three-dimensional and cross-sectional structure of the fabrication was analyzed through confocal microscopy and histological methods, respectively. The BMSCs remained viable and were condensed into many pellet-like cell masses with a diameter ranging from 28.5 to 298.4 (113.5 - 47.9) mm in the OPF scaffold. In vivo osteochondral defect repair was tested in 12 defects created in six 8-month-old Prestige World Genetics micropigs. The implantation of scaffold alone was used for control. Gross morphological, histological, and statistical analyses were performed at 4 months postoperatively. The scaffoldヨMSC treatment led to 99% defect filling, with 84% hyaline-like cartilage at 4 months, which was significantly ( p < 0.0001) more than the 54% neotissue filling and 39% hyaline-like cartilage obtained in the scaffold-only group. The implantation of BMSCs in freeze-dried OPF hydrogel scaffold, which created a conducive environment for cell infiltration and clustering, could fully repair chondral defects with hyaline-like cartilage. This protocol provides a clinically feasible procedure for osteochondral defect treatment.
dc.language.iso eng
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.title Repair of Osteochondral Defects with Rehydrated Freeze-Dried Oligo[Poly(Ethylene Glycol) Fumarate] Hydrogels Seeded with Bone Marrow Mesenchymal Stem Cells in a Porcine Model
dc.type Journal article
dc.citation.journalTitle Tissue Engineering: Part A
dc.contributor.org BioScience Research Collaborative
dc.citation.volumeNumber 19
dc.citation.issueNumber 15-16
dc.contributor.publisher Mary Ann Liebert, Inc.
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1089/ten.tea.2012.0621
dc.identifier.pmid 23517496
dc.type.publication publisher version
dc.citation.firstpage 1852
dc.citation.lastpage 1861


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