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dc.contributor.authorKinard, Lucas A.
Kasper, F. Kurtis
Mikos, Antonios G.
dc.date.accessioned 2013-08-20T22:32:45Z
dc.date.available 2013-08-20T22:32:45Z
dc.date.issued 2012
dc.identifier.citation Kinard, Lucas A., Kasper, F. Kurtis and Mikos, Antonios G.. "Synthesis of oligo(poly(ethylene glycol) fumarate)." Nature Protocols, 7, no. 6 (2012) Nature Publishing Group: 1219-1227. http://dx.doi.org/10.1038/nprot.2012.055.
dc.identifier.urihttps://hdl.handle.net/1911/71749
dc.description.abstract This protocol describes the synthesis of oligo(poly(ethylene glycol) fumarate) (OPF) (1-35 kDa)(a polymer useful for tissue engineering applications) by a one-pot reaction of poly(ethylene glycol) (PEG) and fumaryl chloride. The procedure involves three parts: dichloromethane and PEG are first dried; the reaction step follows in which fumaryl chloride and triethylamine are added dropwise to a solution of PEG in dichloromethane; and finally the product solution is filtered to remove byproduct salt, and the OPF product is twice crystallized, washed, and dried under vacuum. The reaction is affected by PEG molecular weight and reactant molar ratio. The OPF product is cross-linked by radical polymerization by either a thermally induced or UV-induced radical initiator, and the physical properties of the OPF oligomer and resulting cross-linked hydrogel are easily tailored by varying PEG molecular weight. OPF hydrogels are injectable, polymerize in situ, and undergo biodegradation by hydrolysis of ester bonds. The expected time required to complete this protocol is 6 d.
dc.language.iso eng
dc.publisher Nature Publishing Group
dc.rights This is an author's peer-reviewed final manuscript, as accepted by the publisher. The published article is copyrighted by the Nature Publishing Group.
dc.title Synthesis of oligo(poly(ethylene glycol) fumarate)
dc.type Journal article
dc.contributor.funder National Institutes of Health
dc.contributor.funder Armed Forces Institute of Regenerative Medicine
dc.contributor.funder National Science Foundation
dc.citation.journalTitle Nature Protocols
dc.subject.keywordoligo(poly(ethylene glycol) fumarate)
OPF
polymer
tissue engineering
polymer synthesis
radical polymerization
hydrogel
PEG
dc.citation.volumeNumber 7
dc.citation.issueNumber 6
dc.embargo.terms none
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1038/nprot.2012.055
dc.identifier.pmcid PMC3513913
dc.identifier.pmid 22653160
dc.identifier.grantID R01 AR48756 (National Institutes of Health)
dc.identifier.grantID W81XWH-08-2-0032 (Armed Forces Institute of Regenerative Medicine)
dc.identifier.grantID 0940902 (National Science Foundation)
dc.type.publication post-print
dc.citation.firstpage 1219
dc.citation.lastpage 1227


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