Synthesis, Characterization, and Biological uses of Carbon Nanoparticles
Marcano Quevedo, Daniela
Tour, James M.
Doctor of Philosophy
Many diseases have been associated with oxidative stress (OS) which is caused when the production of reactive oxygen species (ROS), such as superoxide (O2•-) and hydroxyl radical (•OH), overcome the scavenging efficiency of living organisms. It is known that ROS production is worsened during traumas related to ischemic events and subsequent reperfusion in which the treatment with fast and effective antioxidants is critical to prevent cell and tissue damage. PEG-HCCs are carbon nanoparticles that showed O2•- and •OH scavenging properties according to electron paramagnetic resonance (EPR) experiments and peroxyl scavenging properties based on oxygen radical absorbance capacity (ORAC) assays. The O2•- quenching capability was also examined in vivo using a mild traumatic brain injury (mTBI) model complicated with hypotension. As result of the PEG-HCCs treatment, the cerebral blood flow (CBF) was restored while normalizing O2•- and nitric oxide (NO•) levels, primarily in the cerebral vasculature
PEG-HCCs; Nanoparticles; Antioxidants; Drug delivery; Graphene oxide