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dc.contributor.authorPunbusayakul, Niramol
Talapatra, Saikat
Ajayan, Pulickel M
Surareungchai, Werasak
dc.date.accessioned 2013-06-19T17:08:05Z
dc.date.available 2013-06-19T17:08:05Z
dc.date.issued 2013
dc.identifier.citation Punbusayakul, Niramol, Talapatra, Saikat, Ajayan, Pulickel M, et al.. "Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay." Chemistry Central Journal, 7, (2013) Chemistry Central: 102. http://dx.doi.org/10.1186/1752-153X-7-102.
dc.identifier.urihttps://hdl.handle.net/1911/71330
dc.description.abstract Background: A label-free immunosensor from as-grown double wall carbon nanotubes (DW) bundles was developed for detecting Salmonella typhimurium. The immunosensor was fabricated by using the as-grown DW bundles as an electrode material with an anti-Salmonella impregnated on the surface. The immunosensor was electrochemically characterized by cyclic voltammetry. The working potential (100, 200, 300 and 400 mV vs. Ag/AgCl) and the anti-Salmonella concentration (10, 25, 50, 75, and 100 μg/mL) at the electrode were subsequently optimized. Then, chronoamperometry was used with the optimum potential of 100 mV vs. Ag/AgCl) and the optimum impregnated anti-Salmonella of 10 μg/mL to detect S. typhimurium cells (0-109 CFU/mL). Results: The DW immunosensor exhibited a detection range of 102 to 107 CFU/mL for the bacteria with a limit of detection of 8.9 CFU/mL according to the IUPAC recommendation. The electrode also showed specificity to S. typhimurium but no current response to Escherichia coli. Conclusions: These findings suggest that the use of a label-free DW immunosensor is promising for detecting S. typhimurium.
dc.language.iso eng
dc.publisher Chemistry Central
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.title Label-free as-grown double wall carbon nanotubes bundles for Salmonella typhimurium immunoassay
dc.type Journal article
dc.citation.journalTitle Chemistry Central Journal
dc.citation.volumeNumber 7
dc.embargo.terms none
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1186/1752-153X-7-102
dc.identifier.pmcid PMC3716848
dc.identifier.pmid 23764320
dc.type.publication publisher version
dc.citation.firstpage 102


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