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dc.contributor.advisor Kiang, Ching-Hwa
dc.creatorFrey, Eric
dc.date.accessioned 2013-06-05T14:54:10Z
dc.date.accessioned 2013-06-05T14:54:22Z
dc.date.available 2013-06-05T14:54:10Z
dc.date.available 2013-06-05T14:54:22Z
dc.date.created 2012-12
dc.date.issued 2013-06-05
dc.date.submitted December 2012
dc.identifier.urihttps://hdl.handle.net/1911/71276
dc.description.abstract Nonequilibrium work theorems, such as the Jarzynski equality and the Crooks fluctuation theorem, allow one to use nonequilibrium measurements to determine equilibrium free energies. For example, it has been demonstrated that the Crooks fluctuation theorem can be used to determine RNA folding energies. We used single-molecule manipulation with an atomic force microscope to measure the work done on poly(dA) as it was stretched and relaxed. This single-stranded nucleic acid exhibits unique base-stacking transitions in its force-extension curve due to the strong interactions among A bases, as well as multiple pathways. Here we showed that free energy curves can be determined by using the Crooks fluctuation theorem. The nonequilibrium work theorem can be used to determine free energy curves even when there are multiple pathways.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectCrooks fluctuation theorem
DNA
Single molecule
Poly(da)
Jarzynski equality
Free energy landscape
dc.title Experimental Free Energy Landscape Reconstruction of DNA Unstacking Using Crooks Fluctuation Theorem
dc.contributor.committeeMember Deem, Michael W.
dc.contributor.committeeMember Nordlander, Peter J.
dc.date.updated 2013-06-05T14:54:22Z
dc.identifier.slug 123456789/ETD-2012-12-210
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Physics and Astronomy
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Science
dc.identifier.citation Frey, Eric. "Experimental Free Energy Landscape Reconstruction of DNA Unstacking Using Crooks Fluctuation Theorem." (2013) Master’s Thesis, Rice University. https://hdl.handle.net/1911/71276.


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