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dc.contributor.authorGonzález, Ramón
dc.date.accessioned 2013-05-22T15:02:32Z
dc.date.available 2013-05-22T15:02:32Z
dc.date.issued 2013
dc.identifier.citation González, Ramón. "Metabolic engineering: Use of system-level approaches and application to fuel production in Escherichia coli." Electronic Journal of Biotechnology, 16, no. 3 (2013) Pontificia Universidad Cat�lica de Valpara�so: http://dx.doi.org/10.2225/vol16-issue3-fulltext-14.
dc.identifier.issn0717-3458
dc.identifier.urihttps://hdl.handle.net/1911/71234
dc.description.abstract Metabolic engineering was formally defined more than two decades ago (Bailey, 1991) and it is now an established discipline. Metabolic engineering is generally defined as the directed improvement of product formation or cellular properties through the modification of specific biochemical reactions or the introduction of new ones with the use of recombinant DNA technology (Bailey, 1991; Stephanopoulos et al. 1998). Therefore, the analysis and engineering/synthesis of metabolic pathways is of central importance to metabolic engineering. The analytical part uses a number of experimental and modeling techniques for the systematic study of cellular responses (in terms of RNA, protein and metabolite levels, metabolic fluxes, etc.) to genetic and environmental perturbations. This facilitates a rational design of metabolic modifications, which are implemented using recombinant DNA technology. Both, the analysis and the synthesis of metabolic pathways will be covered in this review. Recent efforts on the engineering of fermentative and biosynthetic pathways for biofuel production in Escherichia coli, as well as those enabling the utilization of novel feedstocks, will be highlighted.
dc.language.iso eng
dc.publisher Pontificia Universidad Cat�lica de Valpara�so
dc.rights Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
dc.title Metabolic engineering: Use of system-level approaches and application to fuel production in Escherichia coli
dc.type Journal article
dc.contributor.funder National Science Foundation
dc.citation.journalTitle Electronic Journal of Biotechnology
dc.subject.keywordbiofuels
Escherichia coli
metabolic engineering
systems biology
dc.citation.volumeNumber 16
dc.citation.issueNumber 3
dc.embargo.terms none
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.2225/vol16-issue3-fulltext-14
dc.identifier.grantID EEC-0813570 (National Science Foundation)
dc.identifier.grantID CBET-1134541 (National Science Foundation)
dc.identifier.grantID CBET-1067565 (National Science Foundation)
dc.type.publication publisher version


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