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dc.contributor.authorDuman, Joseph G.
Tzeng, Christopher P.
Tu, Yen-Kuei
Munjal, Tina
Schwechter, Brandon
Ho, Tammy Szu-Yu
Tolias, Kimberley F.
dc.date.accessioned 2013-05-02T16:14:27Z
dc.date.available 2014-05-03T05:10:04Z
dc.date.issued 2013
dc.identifier.citation Duman, Joseph G., Tzeng, Christopher P., Tu, Yen-Kuei, et al.. "The Adhesion-GPCR BAI1 Regulates Synaptogenesis by Controlling the Recruitment of the Par3/Tiam1 Polarity Complex to Synaptic Sites." The Journal of Neuroscience, 33, no. 16 (2013) 6964-6978. http://dx.doi.org/10.1523/JNEUROSCI.3978-12.2013.
dc.identifier.urihttps://hdl.handle.net/1911/71082
dc.description.abstract Excitatory synapses are polarized structures that primarily reside on dendritic spines in the brain. The small GTPase Rac1 regulates the development and plasticity of synapses and spines by modulating actin dynamics. By restricting the Rac1-guanine nucleotide exchange factor Tiam1 to spines, the polarity protein Par3 promotes synapse development by spatially controlling Rac1 activation. However, the mechanism for recruiting Par3 to spines is unknown. Here, we identify brain-specific angiogenesis inhibitor 1 (BAI1) as a synaptic adhesion GPCR that is required for spinogenesis and synaptogenesis in mice and rats. We show that BAI1 interacts with Par3/Tiam1 and recruits these proteins to synaptic sites. BAI1 knockdown results in Par3/Tiam1 mislocalization and loss of activated Rac1 and filamentous actin from spines. Interestingly, BAI1 also mediates Rac-dependent engulfment in professional phagocytes through its interaction with a different Rac1-guanine nucleotide exchange factor module, ELMO/DOCK180. However, this interaction is dispensable for BAI1’s role in synapse development because a BAI1 mutant that cannot interact with ELMO/DOCK180 rescues spine defects in BAI1-knockdown neurons, whereas a mutant that cannot interact with Par3/Tiam1 rescues neither spine defects nor Par3 localization. Further, overexpression of Tiam1 rescues BAI1 knockdown spine phenotypes. These results indicate that BAI1 plays an important role in synaptogenesis that is mechanistically distinct from its role in phagocytosis. Furthermore, our results provide the first example of a cell surface receptor that targets members of the PAR polarity complex to synapses.
dc.language.iso eng
dc.title The Adhesion-GPCR BAI1 Regulates Synaptogenesis by Controlling the Recruitment of the Par3/Tiam1 Polarity Complex to Synaptic Sites
dc.type Journal article
dc.citation.journalTitle The Journal of Neuroscience
dc.citation.volumeNumber 33
dc.citation.issueNumber 16
dc.contributor.publisher The Society for Neuroscience
dc.embargo.terms 1 year
dc.type.dcmi Text
dc.identifier.doihttp://dx.doi.org/10.1523/JNEUROSCI.3978-12.2013
dc.citation.firstpage 6964
dc.citation.lastpage 6978


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