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    Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin

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    Author
    Musatov, Andrej; Fabian, Marian
    Date
    2013
    Abstract
    The interaction of peroxidized cardiolipin with ferrocytochrome c induces two kinetically and chemically distinct processes. The first is a rapid oxidation of ferrocytochrome c, followed by a slower, irreversible disruption of heme c. The oxidation of ferrocytochrome c by peroxidized cardiolipin is explained by a Fenton-type reaction. Heme scission is a consequence of the radical-mediated reactions initiated by the interaction of ferric heme iron with peroxidized cardiolipin. Simultaneously with the heme c disruption, generation of hydroxyl radical is detected by EPR spectroscopy using the spin trapping technique. The resulting apocytochrome c sediments as a heterogeneous mixture of high aggregates, as judged by sedimentation analysis. Both the oxidative process and the destructive process were suppressed by nonionic detergents and/or high ionic strength. The mechanism for generating radicals and heme rupture is presented.
    Citation
    Musatov, Andrej and Fabian, Marian. "Elucidating the mechanism of ferrocytochrome c heme disruption by peroxidized cardiolipin." Journal of Biological Inorganic Chemistry, 18, (2013) 137-144. http://dx.doi.org/10.1007/s00775-012-0958-0.
    Published Version
    http://dx.doi.org/10.1007/s00775-012-0958-0
    Keyword
    ferrocytochrome c; peroxidized cardiolipin; heme destruction; hydroxyl radical; aggregation
    Type
    Journal article
    Citable link to this page
    https://hdl.handle.net/1911/70863
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    • Biochemistry and Cell Biology Publications [118]

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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map