Show simple item record

dc.contributor.advisor Braam, Janet
dc.creatorVan Ree, Kalie
dc.date.accessioned 2013-03-08T00:39:47Z
dc.date.available 2013-03-08T00:39:47Z
dc.date.issued 2012
dc.identifier.citation Van Ree, Kalie. "NOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsis." (2012) Master’s Thesis, Rice University. https://hdl.handle.net/1911/70480.
dc.identifier.urihttps://hdl.handle.net/1911/70480
dc.description.abstract Chloroplasts generate important cellular signals and synthesize diverse products. The chloroplast-localized protein, Nitric Oxide Associated-1 (NOA1), is implicated in nitric oxide (NO) accumulation and linked to the methylerythritol phosphate (MEP) pathway, but its role is undefined. I report that NOA1 is not essential for NO accumulation because the noa1 mutant accumulates NO when provided sucrose-supplemented media. Therefore, chloroplast function and fixed carbon, but not NOA1 are likely critical for plant NO accumulation. noa1 is also resistant to fosmidomycin, an inhibitor of the MEP pathway. This phenotype led to uncovering a potential link between the MEP and tetrapyrrole pathways. I report that fosmidomycin toxicity is light dependent and reduced by phytol supplementation. Downregulation of the tetrapyrrole pathway enhances fosmidomycin resistance, suggesting that reduced tetrapyrrole biosynthesis alleviates fosmidomycin toxicity. These findings reveal new insight into how impairment of the MEP pathway affects plants and the importance of metabolic balance for chloroplast function.
dc.format.extent 67 p.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectBiological sciences
Plant biology
dc.title NOA1: A Tool for Understanding Nitric Oxide Accumulation and Fosmidomycin Resistance in Arabidopsis
dc.identifier.digital VanReeK
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biochemistry and Cell Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Arts


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record