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dc.contributor.advisor Matthews, Kathleen S.
dc.contributor.advisor Bondos, Sarah E.
dc.creatorHuang, Zhao
dc.date.accessioned 2013-03-08T00:34:34Z
dc.date.available 2013-03-08T00:34:34Z
dc.date.issued 2011
dc.identifier.citation Huang, Zhao. "Developing Novel Protein-based Materials using Ultrabithorax: Production, Characterization, and Functionalization." (2011) Diss., Rice University. https://hdl.handle.net/1911/70269.
dc.identifier.urihttps://hdl.handle.net/1911/70269
dc.description.abstract Compared to 'conventional' materials made from metal, glass, or ceramics, protein-based materials have unique mechanical properties. Furthermore, the morphology, mechanical properties, and functionality of protein-based materials may be optimized via sequence engineering for use in a variety of applications, including textile materials, biosensors, and tissue engineering scaffolds. The development of recombinant DNA technology has enabled the production and engineering of protein-based materials ex vivo . However, harsh production conditions can compromise the mechanical properties of protein-based materials and diminish their ability to incorporate functional proteins. Developing a new generation of protein-based materials is crucial to (i) improve materials assembly conditions, (ii) create novel mechanical properties, and (iii) expand the capacity to carry functional protein/peptide sequences. This thesis describes development of novel protein-based materials using Ultrabithorax, a member of the Hox family of proteins that regulate developmental pathways in Drosophila melanogaster . The experiments presented (i) establish the conditions required for the assembly of Ubx-based materials, (ii) generate a wide range of Ubx morphologies, (iii) examine the mechanical properties of Ubx fibers, (iv) incorporate protein functions to Ubx-based materials via gene fusion, (v) pattern protein functions within the Ubx materials, and (vi) examine the biocompatibility of Ubx materials in vitro . Ubx-based materials assemble at mild conditions compatible with protein folding and activity, which enables Ubx chimeric materials to retain the function of appended proteins in spatial patterns determined by materials assembly. Ubx-based materials also display mechanical properties comparable to existing protein-based materials and demonstrate good biocompatibility with living cells in vitro . Taken together, this research demonstrates the unique features and future potential of novel Ubx-based materials.
dc.format.extent 179 p.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectApplied sciences
Pure sciences
Biological sciences
Ultrabithorax
Spaital patterning
Self-assembly
Biomaterials
Biochemistry
Nanotechnology
Biophysics
dc.title Developing Novel Protein-based Materials using Ultrabithorax: Production, Characterization, and Functionalization
dc.identifier.digital HuangZ
dc.type.genre Thesis
dc.type.material Text
thesis.degree.department Biochemistry and Cell Biology
thesis.degree.discipline Natural Sciences
thesis.degree.grantor Rice University
thesis.degree.level Doctoral
thesis.degree.name Doctor of Philosophy


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