Now showing items 1-20 of 507

    • Surfactant-assisted individualization and dispersion of boron nitride nanotubes 

      McWilliams, Ashleigh D. Smith; Reyes, Carlos A. de los; Liberman, Lucy; Ergülen, Selin; Talmon, Yeshayahu; Pasquali, Matteo; A. Martí, Angel (2019)
      Boron nitride nanotubes (BNNTs) belong to a novel class of material with useful thermal, electronic and optical properties. However, the study and the development of applications of this material requires the formation of ...
    • A Self-Deleting AAV-CRISPR System for In Vivo Genome Editing 

      Li, Ang; Lee, Ciaran M.; Hurley, Ayrea E.; Jarrett, Kelsey E.; De Giorgi, Marco; Lu, Weiqi; Balderrama, Karol S.; Doerfler, Alexandria M.; Deshmukh, Harshavardhan; Ray, Anirban; Bao, Gang; Lagor, William R. (2019)
      Adeno-associated viral (AAV) vectors packaging the CRISPR-Cas9 system (AAV-CRISPR) can efficiently modify disease-relevant genes in somatic tissues with high efficiency. AAV vectors are a preferred delivery vehicle for ...
    • Clinical training and validation of the LeukoScope: a low-cost, point-of-care device to perform white blood cell and neutrophil counts 

      Majors, Catherine E.; Pawlowski, Michal E.; Burke, Daniel C.; Tkaczyk, Tomasz S.; Rieber, Alyssa; Richards-Kortum, Rebecca (2019)
      A white blood cell (WBC) count with partial differential is an important clinical laboratory test. However, current methods to perform a WBC count and differential are difficult to use at the point of care or too expensive ...
    • Optogenetic control of Bacillus subtilis gene expression 

      Castillo-Hair, Sebastian M.; Baerman, Elliot A.; Fujita, Masaya; Igoshin, Oleg A.; Tabor, Jeffrey J. (2019)
      The Gram-positive bacterium Bacillus subtilis exhibits complex spatial and temporal gene expression signals. Although optogenetic tools are ideal for studying such processes, none has been engineered for this organism. ...
    • A Dye-Free Analog to Retinal Angiography Using Hyperspectral Unmixing to Retrieve Oxyhemoglobin Abundance 

      Dwight, Jason G.; Weng, Christina Y.; Pawlowski, Michal E.; Tkaczyk, Tomasz S. (2019)
      Purpose: Retinal angiography evaluates retinal and choroidal perfusion and vascular integrity and is used to manage many ophthalmic diseases, such as age-related macular degeneration. The most common method, fluorescein ...
    • Highly efficient editing of the β-globin gene in patient-derived hematopoietic stem and progenitor cells to treat sickle cell disease 

      Park, So Hyun; Lee, Ciaran M.; Dever, Daniel P.; Davis, Timothy H.; Camarena, Joab; Srifa, Waracharee; Zhang, Yankai; Paikari, Alireza; Chang, Alicia K.; Porteus, Matthew H.; Sheehan, Vivien A.; Bao, Gang (2019)
      Sickle cell disease (SCD) is a monogenic disorder that affects millions worldwide. Allogeneic hematopoietic stem cell transplantation is the only available cure. Here, we demonstrate the use of CRISPR/Cas9 and a short ...
    • Human genome-edited hematopoietic stem cells phenotypically correct Mucopolysaccharidosis type I 

      Gomez-Ospina, Natalia; Scharenberg, Samantha G.; Mostrel, Nathalie; Bak, Rasmus O.; Mantri, Sruthi; Quadros, Rolen M.; Gurumurthy, Channabasavaiah B.; Lee, Ciaran; Bao, Gang; Suarez, Carlos J.; Khan, Shaukat; Sawamoto, Kazuki; Tomatsu, Shunji; Raj, Nitin; Attardi, Laura D.; Aurelian, Laure; Porteus, Matthew H. (2019)
      Lysosomal enzyme deficiencies comprise a large group of genetic disorders that generally lack effective treatments. A potential treatment approach is to engineer the patient’s own hematopoietic system to express high levels ...
    • Mathematical modelling reveals unexpected inheritance and variability patterns of cell cycle parameters in mammalian cells 

      Mura, Marzena; Feillet, Céline; Bertolusso, Roberto; Delaunay, Franck; Kimmel, Marek (2019)
      The cell cycle is the fundamental process of cell populations, it is regulated by environmental cues and by intracellular checkpoints. Cell cycle variability in clonal cell population is caused by stochastic processes such ...
    • Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER 

      Heins, Zachary J.; Mancuso, Christopher P.; Kiriakov, Szilvia; Wong, Brandon G.; Bashor, Caleb J.; Khalil, Ahmad S. (2019)
      Continuous culture methods enable cells to be grown under quantitatively controlled environmental conditions, and are thus broadly useful for measuring fitness phenotypes and improving our understanding of how genotypes ...
    • Light-guide snapshot imaging spectrometer for remote sensing applications 

      Wang, Ye; Pawlowski, Michal E.; Cheng, Shuna; Dwight, Jason G.; Stoian, Razvan I.; Lu, Jiawei; Alexander, David; Tkaczyk, Tomasz S. (2019)
      A fiber-based snapshot imaging spectrometer was developed with a maximum of 31853 (~188 x 170) spatial sampling and 61 spectral channels in the 450nm-750nm range. A compact, custom-fabricated fiber bundle was used to sample ...
    • Image-based Classification of Tumor Type and Growth Rate using Machine Learning: a preclinical study 

      Tang, Tien T.; Zawaski, Janice A.; Francis, Kathleen N.; Qutub, Amina A.; Gaber, M. Waleed (2019)
      Medical images such as magnetic resonance (MR) imaging provide valuable information for cancer detection, diagnosis, and prognosis. In addition to the anatomical information these images provide, machine learning can ...
    • Evolutionary Relationships Between Low Potential Ferredoxin and Flavodoxin Electron Carriers 

      Campbell, Ian J.; Bennett, George N.; Silberg, Jonathan J. (2019)
      Proteins from the ferredoxin (Fd) and flavodoxin (Fld) families function as low potential electrical transfer hubs in cells, at times mediating electron transfer between overlapping sets of oxidoreductases. To better ...
    • An Engineered B. subtilis Inducible Promoter System with over 10 000-Fold Dynamic Range 

      Castillo-Hair, Sebastian M.; Fujita, Masaya; Igoshin, Oleg A.; Tabor, Jeffrey J. (2019)
      Bacillus subtilis is the leading model Gram-positive bacterium, and a widely used chassis for industrial protein production. However, B. subtilis research is limited by a lack of inducible promoter systems with low leakiness ...
    • Indirect magnetic force microscopy 

      Sifford, Joshua; Walsh, Kevin J.; Tong, Sheng; Bao, Gang; Agarwal, Gunjan (2019)
      Magnetic force microscopy (MFM) is an atomic force microscopy (AFM)-based technique to map magnetic domains in a sample. MFM is widely used to characterize magnetic recording media, magnetic domain walls in materials, ...
    • Rewiring bacterial two-component systems by modular DNA-binding domain swapping 

      Schmidl, Sebastian R.; Ekness, Felix; Sofjan, Katri; Daeffler, Kristina N.-M.; Brink, Kathryn R.; Landry, Brian P.; Gerhardt, Karl P.; Dyulgyarov, Nikola; Sheth, Ravi U.; Tabor, Jeffrey J. (2019)
      Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways and valuable sensors for synthetic biology. However, most TCSs remain uncharacterized or difficult to harness for applications. ...
    • Gene correction for SCID-X1 in long-term hematopoietic stem cells 

      Pavel-Dinu, Mara; Wiebking, Volker; Dejene, Beruh T.; Srifa, Waracharee; Mantri, Sruthi; Nicolas, Carmencita E.; Lee, Ciaran; Bao, Gang; Kildebeck, Eric J.; Punjya, Niraj; Sindhu, Camille; Inlay, Matthew A.; Saxena, Nivedita; DeRavin, Suk See; Malech, Harry; Roncarolo, Maria Grazia; Weinberg, Kenneth I.; Porteus, Matthew H. (2019)
      Gene correction in human long-term hematopoietic stem cells (LT-HSCs) could be an effective therapy for monogenic diseases of the blood and immune system. Here we describe an approach for X-linked sSevere cCombined ...
    • Collagen-rich airway smooth muscle cells are a metastatic niche for tumor colonization in the lung 

      Lee, Yu-Cheng; Kurtova, Antonina V.; Xiao, Jing; Nikolos, Fotis; Hayashi, Kazukuni; Tramel, Zoe; Jain, Antrix; Chen, Fengju; Chokshi, Mithil; Lee, Ciaran; Bao, Gang; Zhang, Xiang; Shen, Jianjun; Mo, Qianxing; Jung, Sung Yun; Rowley, David; Chan, Keith Syson (2019)
      Metastases account for the majority of cancer deaths. While certain steps of the metastatic cascade are well characterized, identification of targets to block this process remains a challenge. Host factors determining ...
    • Low-Cost Instructional Apparatus to Improve Training for Cervical Cancer Screening and Prevention 

      Parra, Sonia; Oden, Maria; Schmeler, Kathleen; Richards-Kortum, Rebecca; Rice360 Student Team (2019)
      BACKGROUND: Cervical cancer remains one of the leading causes of cancer for women in medically underserved areas. This is in part due to a lack of trained clinicians to provide the necessary diagnosis and treatment of ...
    • Biophysics at the coffee shop: lessons learned working with George Oster 

      Igoshin, Oleg A.; Chen, Jing; Xing, Jianhua; Liu, Jian; Elston, Timothy C.; Grabe, Michael; Kim, Kenneth S.; Nirody, Jasmine A.; Rangamani, Padmini; Sun, Sean X.; Wang, Hongyun; Wolgemuth, Charles (2019)
      Over the past 50 years, the use of mathematical models, derived from physical reasoning, to describe molecular and cellular systems has evolved from an art of the few to a cornerstone of biological inquiry. George Oster ...