Now showing items 92-111 of 115

    • Structural Basis of Stereospecificity in the Bacterial Enzymatic Cleavage of β-Aryl Ether Bonds in Lignin 

      Helmich, Kate E.; Pereira, Jose Henrique; Gall, Daniel L.; Heins, Richard A.; McAndrew, Ryan P.; Bingman, Craig; Deng, Kai; Holland, Keefe C.; Noguera, Daniel R.; Simmons, Blake A.; Sale, Kenneth L.; Ralph, John; Donohue, Timothy J.; Adams, Paul D.; Phillips, George N. Jr. (2016)
      Lignin is a combinatorial polymer comprising monoaromatic units that are linked via covalent bonds. Although lignin is a potential source of valuable aromatic chemicals, its recalcitrance to chemical or biological digestion ...
    • Structure analysis of free and bound states of an RNA aptamer against ribosomal protein S8 from Bacillus anthracis 

      Davlieva, Milya; Donarski, James; Wang, Jiachen; Shamoo, Yousif; Nikonowicz, Edward P. (2014)
      Several protein-targeted RNA aptamers have been identified for a variety of applications and although the affinities of numerous protein-aptamer complexes have been determined, the structural details of these complexes ...
    • Structure and assembly of the influenza A virus ribonucleoprotein complex 

      Zheng, Wenj; Tao, Yizhi Jane (2013)
      The genome of influenza A viruses consists of eight segments of single-stranded, negative-sense RNA that are encapsidated as individual rod-shaped ribonucleoprotein complexes (RNPs). Each RNP contains a viral RNA, a viral ...
    • Structure of androcam supports specialized interactions with myosin VI 

      Joshi, Mehul K.; Moran, Sean; Beckingham, Kathleen M.; MacKenzie, Kevin R. (2012)
      Androcam replaces calmodulin as a tissue-specific myosin VI light chain on the actin cones that mediate D. melanogaster spermatid individualization. We show that the androcam structure and its binding to the myosin VI ...
    • Structure, Dynamics, and Specificity of Endoglucanase D from Clostridium cellulovorans 

      Bianchetti, Christopher M.; Brumm, Phillip; Smith, Robert W.; Dyer, Kevin; Hura, Greg L.; Rutkoski, Thomas J.; Phillips, George N. Jr. (2013)
      The enzymatic degradation of cellulose is a critical step in the biological conversion of plant biomass into an abundant renewable energy source. An understanding of the structural and dynamic features that cellulases ...
    • Structure-Guided Functional Characterization of Enediyne Self-Sacrifice Resistance Proteins, CalU16 and CalU19 

      Elshahawi, Sherif I.; Ramelot, Theresa A.; Seetharaman, Jayaraman; Chen, Jing; Singh, Shanteri; Yang, Yunhuang; Pederson, Kari; Kharel, Madan K.; Xiao, Rong; Lew, Scott; Yennamalli, Ragothaman M.; Miller, Mitchell D.; Wang, Fengbin; Tong, Liang; Montelione, Gaetano T.; Kennedy, Michael A.; Bingman, Craig A.; Zhu, Haining; Phillips, George N. Jr.; Thorson, Jon S. (2014)
      Calicheamicin γ1I (1) is an enediyne antitumor compound produced by Micromonospora echinospora spp. calichensis, and its biosynthetic gene cluster has been previously reported. Despite extensive analysis and biochemical ...
    • Synthetic biology: the many facets of T7 RNA polymerase 

      Shis, David L.; Bennett, Matthew R. (2014)
      Split T7 RNA polymerase provides new avenues for creating synthetic gene circuits that are decoupled from host regulatory processesラbut how many times can this enzyme be split, yet retain function? New research by Voigt ...
    • A Technique to Increase Accessibility to Late-Stage Chick Embryos for In Ovo Manipulations 

      Spurlin, James III; Lwigale, Peter (2013)
      Background: During early development, avian embryos are easily accessible in ovo for transplantations and experimental perturbations. However, these qualities of the avian embryonic model rapidly wane shortly after embryonic ...
    • TFEB regulates lysosomal proteostasis 

      Song, Wensi; Wang, Fan; Savini, Marzia; Ake, Ashley; di Ronza, Alberto; Sardiello, Marco; Segatori, Laura (2013)
      Loss-of-function diseases are often caused by destabilizing mutations that lead to protein misfolding and degradation. Modulating the innate protein homeostasis (proteostasis) capacity may lead to rescue of native folding ...
    • The crystal structure of BlmI as a model for nonribosomal peptide synthetase peptidyl carrier proteins 

      Lohman, Jeremy R.; Ma, Ming; Cuff, Marianne E.; Bigelow, Lance; Bearden, Jessica; Babnigg, Gyorgy; Joachimiak, Andrzej; Phillips, George N. Jr.; Shen, Ben (2014)
      Carrier proteins (CPs) play a critical role in the biosynthesis of various natural products, especially in nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzymology, where the CPs are referred to as ...
    • Three-dimensional in vitro tumor models for cancer research and drug evaluation 

      Xu, Xian; Farach-Carson, Mary C.; Jia, Xinqiao (2014)
      Cancer occurs when cells acquire genomic instability and inflammation, produce abnormal levels of epigenetic factors/proteins and tumor suppressors, reprogram the energy metabolism and evade immune destruction, leading to ...
    • Tissue engineering perfusable cancer models 

      Fong, E.L.; Santoro, M.; Farach-Carson, M.C.; Kasper, F.K.; Mikos, A.G. (2014)
      The effect of fluid flow on cancer progression is currently not well understood, highlighting the need for perfused tumor models to close this gap in knowledge. Enabling biological processes at the cellular level to be ...
    • Toll Mediated Infection Response Is Altered by Gravity and Spaceflight in Drosophila 

      Taylor, Katherine; Kleinhesselink, Kurt; George, Michael D.; Morgan, Rachel; Smallwood, Tangi; Hammonds, Ann S.; Fuller, Patrick M.; Saelao, Perot; Alley, Jeff; Gibbs, Allen G.; Hoshizaki, Deborah K.; von Kalm, Laurence; Fuller, Charles A.; Beckingham, Kathleen M.; Kimbrell, Deborah A. (2014)
      Space travel presents unlimited opportunities for exploration and discovery, but requires better understanding of the biological consequences of long-term exposure to spaceflight. Immune function in particular is relevant ...
    • Total Synthesis of Viridicatumtoxin B and Analogues Thereof: Strategy Evolution, Structural Revision, and Biological Evaluation 

      Nicolaou, K.C.; Hale, Christopher R.H.; Nilewski, Christian; Ioannidou, Heraklidia A.; ElMarrouni, Abdelatif; Nilewski, Lizanne G.; Beabout, Kathryn; Wang, Tim T.; Shamoo, Yousif (2014)
      The details of the total synthesis of viridicatumtoxin B (1) are described. Initial synthetic strategies toward this intriguing tetracycline antibiotic resulted in the development of key alkylation and Lewis acid-mediated ...
    • Toxicity of Quantum Dots and Cadmium Salt to Caenorhabditis elegans after Multigenerational Exposure 

      Contreras, Elizabeth Q.; Cho, Minjung; Zhu, Huiguang; Puppala, Hema L.; Escalera, Gabriela; Zhong, Weiwei; Colvin, Vicki L. (2013)
      To fully understand the biological and environmental impacts of nanomaterials requires studies that address both sublethal end points and multigenerational effects. Here, we use a nematode to examine these issues as they ...
    • Transcriptional Activation by NFκB Increases Perlecan/HSPG2 Expression in the Desmoplastic Prostate Tumor Microenvironment 

      Warren, Curtis R.; Grindel, Brian J.; Francis, Lewis; Carson, Daniel D.; Farach-Carson, Mary C. (2014)
      Perlecan/HSPG2, a heparan sulfate proteoglycan typically found at tissue borders including those separating epithelia and connective tissue, increases near sites of invasion of primary prostatic tumors as previously shown ...
    • Transcriptional Delay Stabilizes Bistable Gene Networks 

      Gupta, Chinmaya; Lopez, Jose Manuel; Ott, William; Josic, Kresimir; Bennett, Matthew R. (2013-08)
      Transcriptional delay can significantly impact the dynamics of gene networks. Here we examine how such delay affects bistable systems. We investigate several stochastic models of bistable gene networks and find that ...
    • A tRNA splicing operon: Archease endows RtcB with dual GTP/ATP cofactor specificity and accelerates RNA ligation 

      Desai, Kevin K.; Cheng, Chin L.; Bingman, Craig A.; Phillips, George N. Jr.; Raines, Ronald T. (2014)
      Archease is a 16-kDa protein that is conserved in all three domains of life. In diverse bacteria and archaea, the genes encoding Archease and the tRNA ligase RtcB are localized into an operon. Here we provide a rationale ...
    • Tunable Protease-Activatable Virus Nanonodes 

      Judd, Justin; Ho, Michelle L.; Tiwari, Abhinav; Gomez, Eric J.; Dempsey, Christopher; Vliet, Kim Van; Igoshin, Oleg A.; Silberg, Jonathan J.; Agbandje-McKenna, Mavis; Suh, Junghae (2014)
      We explored the unique signal integration properties of the self-assembling 60-mer protein capsid of adeno-associated virus (AAV), a clinically proven human gene therapy vector, by engineering proteolytic regulation of ...
    • Understanding molecular recognition of promiscuity of thermophilic methionine adenosyltransferase sMAT from Sulfolobus solfataricus 

      Wang, Fengbin; Singh, Shanteri; Zhang, Jianjun; Huber, Tyler D.; Helmich, Kate E.; Sunkara, Manjula; Hurley, Katherine A.; Goff, Randal D.; Bingman, Craig A.; Morris, Andrew J.; Thorson, Jon S.; Phillips, George N. Jr. (2014)