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dc.contributor.advisor San, Ka-Yiu
dc.creatorJan, Joanna
dc.date.accessioned 2012-09-06T04:18:07Z
dc.date.accessioned 2012-09-06T04:18:10Z
dc.date.available 2012-09-06T04:18:07Z
dc.date.available 2012-09-06T04:18:10Z
dc.date.created 2012-05
dc.date.issued 2012-09-05
dc.date.submitted May 2012
dc.identifier.citation Jan, Joanna. "Metabolic Engineering and Transhydrogenase Effects on NADPH Availability in Escherichia coli." (2012) Master’s Thesis, Rice University. https://hdl.handle.net/1911/64664.
dc.identifier.urihttps://hdl.handle.net/1911/64664
dc.description.abstract The ultimate goal in the field of metabolic engineering is improving cellular processes in a rational manner using engineering design principles and molecular biology techniques. The syntheses of several industrially useful compounds are cofactor-dependent. The reducing equivalent NADPH is required in several enzymatic reactions leading up to the synthesis of high-value compounds like polymers, chiral alcohols, and antibiotics. However, it’s a highly costly compound with limited intracellular availability. This study focuses on the genetic manipulation of a whole-cell system using the two transhydrogenase isoforms pntAB and udhA. Two model systems are used: 1) the production of (S)-2-chloropropionate and 2) the production of poly(3-hydroxybutyrate). Results suggest that the presence of udhA increases product yield and NADPH availability while the presence of pntAB has the opposite effect. A maximum product yield of 1.4 mole-product/mole-glucose was achieved aerobically in a pntAB-deletion strain with udhA overexpression, a 150% improvement over the wild-type control strain.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.subjectMetabolic engineering
Escherichia coli
Nadph
Cofactor
Transhydrogenase
dc.title Metabolic Engineering and Transhydrogenase Effects on NADPH Availability in Escherichia coli
dc.type Thesis
dc.contributor.committeeMember Bennett, George N.
dc.contributor.committeeMember Gonzalez, Ramon
dc.date.updated 2012-09-06T04:18:10Z
dc.identifier.slug 123456789/ETD-2012-05-127
dc.type.material Text
thesis.degree.department Bioengineering
thesis.degree.discipline Engineering
thesis.degree.grantor Rice University
thesis.degree.level Masters
thesis.degree.name Master of Science


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