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    Metabolic Engineering and Transhydrogenase Effects on NADPH Availability in Escherichia coli

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    Author
    Jan, Joanna
    Date
    2012-09-05
    Advisor
    San, Ka-Yiu
    Degree
    Master of Science
    Abstract
    The ultimate goal in the field of metabolic engineering is improving cellular processes in a rational manner using engineering design principles and molecular biology techniques. The syntheses of several industrially useful compounds are cofactor-dependent. The reducing equivalent NADPH is required in several enzymatic reactions leading up to the synthesis of high-value compounds like polymers, chiral alcohols, and antibiotics. However, it’s a highly costly compound with limited intracellular availability. This study focuses on the genetic manipulation of a whole-cell system using the two transhydrogenase isoforms pntAB and udhA. Two model systems are used: 1) the production of (S)-2-chloropropionate and 2) the production of poly(3-hydroxybutyrate). Results suggest that the presence of udhA increases product yield and NADPH availability while the presence of pntAB has the opposite effect. A maximum product yield of 1.4 mole-product/mole-glucose was achieved aerobically in a pntAB-deletion strain with udhA overexpression, a 150% improvement over the wild-type control strain.
    Keyword
    Metabolic engineering; Escherichia coli; Nadph; Cofactor; Transhydrogenase
    Citation
    Jan, Joanna. "Metabolic Engineering and Transhydrogenase Effects on NADPH Availability in Escherichia coli." (2012) Master’s Thesis, Rice University. https://hdl.handle.net/1911/64664.
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    Home | FAQ | Contact Us | Privacy Notice | Accessibility Statement
    Managed by the Digital Scholarship Services at Fondren Library, Rice University
    Physical Address: 6100 Main Street, Houston, Texas 77005
    Mailing Address: MS-44, P.O.BOX 1892, Houston, Texas 77251-1892
    Site Map